Centre of New Technologies, University of Warsaw, Banacha 2C, 02-097, Warsaw, Poland.
Eur Biophys J. 2023 Oct;52(6-7):533-544. doi: 10.1007/s00249-023-01673-w. Epub 2023 Aug 23.
Peptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. PNA sequences can be designed to selectively silence gene expression, which makes PNA a promising tool for antimicrobial applications. However, the poor membrane permeability of PNA remains the main limiting factor for its applications in cells. To overcome this obstacle, PNA conjugates with different molecules have been developed. This mini-review focuses on covalently linked conjugates of PNA with cell-penetrating peptides, aminosugars, aminoglycoside antibiotics, and non-peptidic molecules that were tested, primarily as PNA carriers, in antibacterial and antiviral applications. The chemistries of the conjugation and the applied linkers are also discussed.
肽核酸 (PNA) 是一种核酸类似物，对天然 DNA 或 RNA 具有高度特异性和结合亲和力，并且具有抗酶降解的能力。PNA 序列可以被设计为选择性地沉默基因表达，这使得 PNA 成为一种有前途的抗菌应用工具。然而，PNA 的细胞膜通透性差仍然是其在细胞中应用的主要限制因素。为了克服这一障碍，已经开发出与不同分子连接的 PNA 缀合物。本篇迷你综述主要关注与细胞穿透肽、氨基糖、氨基糖苷抗生素和非肽类分子共价连接的 PNA 缀合物，这些缀合物在抗菌和抗病毒应用中主要被用作 PNA 载体进行了测试。还讨论了缀合的化学和应用的连接子。
