Cruz-Orive L M, Hunziker E B
J Microsc. 1986 Jul;143(Pt 1):47-80. doi: 10.1111/j.1365-2818.1986.tb02765.x.
A number of either new or recently available stereological methods are described for estimating volume, surface area and number of anisotropic cells. The methods are illustrated with direct reference to the epiphyseal growth plate. Different estimates of a given quantity are obtained by applying alternative methods to the same set of sections, in order to compare the relative merits of the methods. For instance, the surface area of the cells is estimated via the Dimroth-Watson model (which gives a measure of the degree of anisotropy in addition to the surface area estimate) and from vertical sections using cycloid test systems. Cell number is estimated by traditional unfolding methods and by the new disector method. Also, volume-weighted mean cell volume is estimated from vertical sections via point-sampled intercepts using two different kinds of rulers to classify intercept lengths. Finally, nested design statistics is applied to a set of data from twelve animals in order to compare the relative impacts of biological and stereological (sampling) variations on the observed coefficient of error of a group mean estimate. The preferred methods are listed in the final section.
本文描述了一些新的或最近可用的体视学方法，用于估计各向异性细胞的体积、表面积和数量。文中以骨骺生长板为例对这些方法进行了说明。通过将不同的方法应用于同一组切片来获得给定数量的不同估计值，以便比较这些方法的相对优缺点。例如，细胞表面积通过Dimroth-Watson模型（除了表面积估计外，还给出各向异性程度的度量）以及使用摆线测试系统从垂直切片进行估计。细胞数量通过传统的展开法和新的分割体法进行估计。此外，体积加权平均细胞体积通过使用两种不同的尺子对截距长度进行分类，从垂直切片通过点采样截距进行估计。最后，将嵌套设计统计应用于来自12只动物的一组数据，以比较生物学变异和体视学（抽样）变异对观察到的组均值估计误差系数的相对影响。最后一部分列出了首选方法。
