Hoefsmit E C, Korn C, Blijleven N, Ploem J S
J Microsc. 1986 Aug;143(Pt 2):161-9. doi: 10.1111/j.1365-2818.1986.tb02774.x.
To detect plasma membrane antigens, cytocentrifuge preparations of the macrophage-like cell line P388D1 were incubated with monoclonal antibodies and labelled with 5 and 20 nm gold particles conjugated to immunoglobulins or protein A. The 20 nm, but not the 5 nm, particles could be observed by reflection-contrast light microscopy. Single 5 nm particles, however, were clearly demonstrable with silver contrast enhancement, both in absorption contrast and in reflection contrast. The method proved to be compatible with cytochemical methods such as acid phosphatase.
为检测质膜抗原，将巨噬细胞样细胞系P388D1的细胞离心涂片与单克隆抗体孵育，并用与免疫球蛋白或蛋白A偶联的5纳米和20纳米金颗粒进行标记。通过反射对比光学显微镜可以观察到20纳米的颗粒，但观察不到5纳米的颗粒。然而，通过银增强对比，无论是吸收对比还是反射对比，都能清楚地显示单个5纳米的颗粒。该方法被证明与诸如酸性磷酸酶等细胞化学方法兼容。
