Jiang Nanfang, Jin Lei, Li Shuyu
Department of Gastroenterology, Hubei No. 3 People's Hospital of Jianghan University, Wuhan, Hubei 430030, P.R. China.
Oncol Lett. 2023 Aug 7;26(3):411. doi: 10.3892/ol.2023.13997. eCollection 2023 Sep.
Recently, the incidence rate of digestive system tumors has increased in China and these tumors occur in a younger population. The present study aimed to determine the expression levels and potential clinical value of secreted phosphoprotein 1 (SPP1) in gastrointestinal cancer. The microarray datasets GSE104836, GSE189830 and GSE103236, obtained from the gene expression omnibus database, were analyzed to determine differentially expressed genes in patients with colorectal cancer (CRC), gastric cancer (GC) and esophageal cancer (EC). A total of 42 patients with CRC, GC or EC and 21 healthy controls were recruited to obtain blood and tissues samples. SPP1 expression levels were detected using reverse transcription-quantitative PCR. Moreover, levels of significance of SPP1 in patients with CRC, GC and EC were analyzed using receiver operating characteristic analysis. Potential correlations between SPP1 and carcinoembryonic antigen (CEA) were assessed using Pearson's correlation coefficient. SPP1 was significantly upregulated in the serum, plasma and tissue of patients with CRC, GC or EC. In addition, the area under the curve of SPP1 was >0.5 in the plasma, serum and cancer tissue of patients with early and late CRC, GC or EC. The present study further demonstrated that the specificity and sensitivity of SPP1 was higher in patients with late CRC, GC or EC compared with patients with early CRC, GC or EC. Moreover, SPP1 and CEA were significantly positively correlated in serum of patients with CRC, GC or EC. In conclusion, the current study demonstrated that SPP1 exhibited significant diagnostic value for gastrointestinal tumors, which suggested that SPP1 may exhibit potential as a diagnostic marker of CRC, GC and EC. The present study provided a novel theoretical basis for the role of SPP1 as a diagnostic marker of digestive system tumors.
近年来，中国消化系统肿瘤的发病率呈上升趋势，且这些肿瘤在较年轻人群中出现。本研究旨在确定分泌型磷蛋白1（SPP1）在胃肠道癌中的表达水平及潜在临床价值。对从基因表达综合数据库获得的微阵列数据集GSE104836、GSE189830和GSE103236进行分析，以确定结直肠癌（CRC）、胃癌（GC）和食管癌（EC）患者中的差异表达基因。共招募了42例CRC、GC或EC患者及21名健康对照，获取血液和组织样本。采用逆转录定量PCR检测SPP1表达水平。此外，使用受试者工作特征分析来分析SPP1在CRC、GC和EC患者中的显著性水平。使用Pearson相关系数评估SPP1与癌胚抗原（CEA）之间的潜在相关性。SPP1在CRC、GC或EC患者的血清、血浆和组织中显著上调。此外，在早期和晚期CRC、GC或EC患者的血浆、血清和癌组织中，SPP1的曲线下面积均>0.5。本研究进一步表明，与早期CRC、GC或EC患者相比，晚期CRC、GC或EC患者中SPP1的特异性和敏感性更高。此外,在CRC、GC或EC患者的血清中，SPP1与CEA显著正相关。总之，本研究表明SPP1对胃肠道肿瘤具有显著的诊断价值，这表明SPP1可能具有作为CRC、GC和EC诊断标志物的潜力。本研究为SPP1作为消化系统肿瘤诊断标志物的作用提供了新的理论依据。
