Ito H, Shimura K
Jpn J Pharmacol. 1986 Jul;41(3):307-14. doi: 10.1254/jjp.41.307.
The antitumor effects of Xiao-Chai-Hu-Tang (Shosaiko-To: SHX) with or without 5-fluorouracil (5-FU) or cyclophosphamide (CY) were examined in an experimental system of lung metastasis induced by Lewis lung carcinoma in C57BL/6crSlc mice. Lewis lung carcinoma cells were implanted into the footpads of the mice. Ten days later, the implanted tumors were surgically removed. The effects of SHX were evaluated by the number of lung surface nodules present 14 days after removal of the implanted tumor. The administration of SHX, p.o. (300 mg/kg X 2/day X 10) caused the antimetastatic effect. Therapy with SHX plus 5-FU or CY significantly inhibited the development of lung metastases. The number of peritoneal macrophages and the degree of the binding of C3 cleavage products (C3b) to macrophages were enhanced in the mice treated with SHX. Lung metastases were inhibited by the i.v. administration of peritoneal macrophages activated with SHX, p.o. These findings raise the possibility that SHX may have clinical value in the prevention of cancer metastasis.
在C57BL/6crSlc小鼠Lewis肺癌诱导的肺转移实验系统中，研究了小柴胡汤（Shosaiko-To: SHX）联合或不联合5-氟尿嘧啶（5-FU）或环磷酰胺（CY）的抗肿瘤作用。将Lewis肺癌细胞接种到小鼠的足垫中。10天后，手术切除植入的肿瘤。通过切除植入肿瘤14天后肺表面结节的数量来评估SHX的作用。口服SHX（300mg/kg×2/天×10）具有抗转移作用。SHX联合5-FU或CY治疗可显著抑制肺转移的发生。在用SHX治疗的小鼠中，腹腔巨噬细胞的数量以及C3裂解产物（C3b）与巨噬细胞的结合程度均有所增强。静脉注射经口服SHX激活的腹腔巨噬细胞可抑制肺转移。这些发现提示SHX在预防癌症转移方面可能具有临床价值。
