Department of General and Analytical Chemistry, Medical University of Silesia, Jagiellońska 4, 41-200, Sosnowiec, Poland.
Department of General and Analytical Chemistry, Medical University of Silesia, Jagiellońska 4, 41-200, Sosnowiec, Poland.
Chemosphere. 2023 Nov;341:139854. doi: 10.1016/j.chemosphere.2023.139854. Epub 2023 Aug 22.
The aim of the work was primarily to determine the relationship between the doses of Fenton's reagents and the effectiveness of the ecotoxicity removal of aqueous solutions containing selected antibiotics. The degradation process of ampicillin, doxycycline, and tylosin in an acidic environment in the presence of HO and FeSO was studied. The effect of reagent doses on the degree of degradation and identification of antibiotic transformation products was measured by the UPLC qTOF method. The degree of mineralisation was determined based on changes in the concentration of total organic carbon. The ecotoxicity of products was determined with commercial MARA® and MICROTOX® bioassays, as well as against unselected microorganisms from polluted rivers and wastewater treatment plant effluent. It was found that the complete degradation of antibiotics and the simultaneous elimination of the toxicity of the Fenton process products required the use of a precisely defined amount of reagents. When an insufficient dose of reactants was used, the post-reaction solutions contained antibiotic derivatives showing antimicrobial activity. On the other hand, the toxicity of the post-reaction solution against to microbiocenoses was observed when too high doses of HO were used in the process. This effect resulted from the presence of unreacted reagent or other unidentified peroxides.
这项工作的主要目的是确定芬顿试剂剂量与含选定抗生素水溶液的生态毒性去除效果之间的关系。在 HO 和 FeSO 的存在下，研究了酸性环境中氨苄青霉素、强力霉素和泰乐菌素的降解过程。通过 UPLC qTOF 方法测量了试剂剂量对降解程度和抗生素转化产物鉴定的影响。基于总有机碳浓度的变化来确定矿化程度。通过商业 MARA®和 MICROTOX®生物测定法以及对受污染河流和废水处理厂出水的未选择的微生物来测定产物的生态毒性。结果发现，完全降解抗生素并同时消除芬顿工艺产物的毒性需要使用精确定义的试剂量。当反应物的剂量不足时，反应后的溶液中含有表现出抗菌活性的抗生素衍生物。另一方面，当过程中使用过高剂量的 HO 时，反应后溶液对微生物群落的毒性也会被观察到。这种效应是由于未反应的试剂或其他未识别的过氧化物的存在所致。
