Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University, Chengdu, China.
Department of Microbiology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
Helicobacter. 2023 Dec;28(6):e13016. doi: 10.1111/hel.13016. Epub 2023 Aug 25.
Helicobacter pylori is one of the most common chronic bacterial infections. Active eradication of H. pylori infection is rare due to the fact that most infected patients are asymptomatic and the use of large amounts of antibiotics in eradication therapy leads to severe side effects. Urolithin B (UB) is an additional major intestinal metabolite of ellagic acid (EA), which has been shown to possess anti-inflammatory, antioxidant, and antiapoptotic biological activities. Preventing the incidence of H. pylori-related gastric disease and reducing the damage to the host by H. pylori is a current approach to control H. pylori infection. In this study, we explored the effect of UB on H. pylori infection.
The effects of UB on inflammation and oxidative stress induced by H. pylori in vivo and in vitro were investigated by qPCR, ELISA, HE staining, IHC staining, etc. RESULTS: UB reduced the adhesion and colonization of H. pylori and improved H. pylori-induced inflammation and oxidative stress in vivo and in vitro. Moreover, UB had better anti-inflammatory and antioxidant effects than clarithromycin (CLR) and metronidazole (MET). In addition to inhibiting the secretion of CagA, UB reduced tissue damage by H. pylori infection.
UB was effective in improving damage caused by H. pylori.
幽门螺杆菌是最常见的慢性细菌感染之一。由于大多数感染患者无症状，且根除治疗中大量使用抗生素会导致严重的副作用，因此，主动根除幽门螺杆菌感染的情况较为少见。鞣花酸（EA）的另一种主要肠道代谢物是尿石素 B（UB），已证明其具有抗炎、抗氧化和抗细胞凋亡的生物学活性。预防与幽门螺杆菌相关的胃病的发生和减少幽门螺杆菌对宿主的损害是目前控制幽门螺杆菌感染的方法。在本研究中，我们探讨了 UB 对幽门螺杆菌感染的影响。
通过 qPCR、ELISA、HE 染色、免疫组化染色等方法研究 UB 对体内和体外幽门螺杆菌感染引起的炎症和氧化应激的影响。
UB 减少了幽门螺杆菌的黏附和定植，并改善了体内和体外幽门螺杆菌诱导的炎症和氧化应激。此外，UB 比克拉霉素（CLR）和甲硝唑（MET）具有更好的抗炎和抗氧化作用。除了抑制 CagA 的分泌外，UB 还减轻了幽门螺杆菌感染引起的组织损伤。
UB 能有效改善幽门螺杆菌引起的损伤。
