Shostak Kristina, González-Peña Fundora Dianevys, Blackman Christopher, Witte Tom, Sproule Amanda, Overy David, Eranthodi Anas, Thakor Nehal, Foroud Nora A, Subramaniam Rajagopal
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.
Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada.
J Fungi (Basel). 2023 Aug 2;9(8):816. doi: 10.3390/jof9080816.
Genetic studies have shown that the MAP kinase MGV1 and the transcriptional regulator TRI6 regulate many of the same biosynthetic gene clusters (BGCs) in . This study sought to investigate the relationship between and in the regulatory hierarchy. Transgenic strains constitutively expressing and were generated to address both independent and epistatic regulation of BGCs by and . We performed a comparative transcriptome analysis between axenic cultures grown in nutrient-rich and secondary metabolite-inducing conditions. The results indicated that BGCs regulated independently by included genes of BGC52, whereas genes uniquely regulated by included the gene cluster (BGC49) that produces gramillin. To understand the epistatic relationship between and , CRISPR/Cas9 was used to insert a constitutive promoter to drive expression in the Δ strain. The results indicate that BGCs that produce deoxynivalenol and fusaoctaxin are co-regulated, with being partially regulated by . Overall, the findings from this study indicate that MGV1 provides an articulation point to differentially regulate various BGCs. Moreover, TRI6, embedded in one of the BGCs provides specificity to regulate the expression of the genes in the BGC.
遗传研究表明，丝裂原活化蛋白激酶MGV1和转录调节因子TRI6在……中调节许多相同的生物合成基因簇（BGCs）。本研究旨在探究……在调控层次结构中的关系。构建了组成型表达……和……的转基因……菌株，以研究……和……对BGCs的独立调控和上位调控。我们对在营养丰富和次级代谢产物诱导条件下生长的无菌培养物进行了比较转录组分析。结果表明，由……独立调控的BGCs包括BGC52的基因，而由……独特调控的基因包括产生gramillin的基因簇（BGC49）。为了了解……和……之间的上位关系，使用CRISPR/Cas9在Δ……菌株中插入一个组成型启动子来驱动……表达。结果表明，产生脱氧雪腐镰刀菌烯醇和fusaoctaxin的BGCs是共同调控的，……部分受……调控。总体而言，本研究结果表明MGV1提供了一个衔接点来差异调节各种BGCs。此外，嵌入其中一个BGCs的TRI6为调节BGCs中基因的表达提供了特异性。
