Boyno Gökhan, Demir Semra, Rezaee Danesh Younes, Durak Emre Demirer, Çevik Rojbin, Farda Beatrice, Djebaili Rihab, Pellegrini Marika
Department of Plant Protection, Faculty of Agriculture, Van Yuzuncu Yil University, Van 65090, Türkiye.
Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia 5756151818, Iran.
J Fungi (Basel). 2023 Aug 14;9(8):845. doi: 10.3390/jof9080845.
Monitoring the dynamics of the spore bank of arbuscular mycorrhizal fungi (AMF) is essential for the sustainable management and protection of agroecosystems. The most common method for extracting AMF spores from soil is the wet-sieving technique (WST). However, this method has many disadvantages. In this study, we modified the WST using new approaches: the ultrasound wet-sieving technique (UWST) and the ultrasound centrifuge technique (UCT). We enumerated and compared the numbers and quality of spores obtained from WST, UWST, and UCT to validate the new modified techniques. We extracted AMF spores from the rhizospheres of different plants, including wheat ( L.), bean ( L.), tomato ( L.), pepper ( L.), parsley ( Mill.), and turfgrass ( L.) collected from the Van Lake basin, Turkey. The highest and lowest AMF spore numbers were observed in wheat and turfgrass rhizospheres. The UCT allowed for the extraction of the highest number of spores from all rhizospheres, followed by the UWST and WST. The UWST and WST allowed for the extraction of similar spore numbers from wheat, pepper, parsley, and turfgrass rhizospheres. Beyond the high extracted spore number, UCT was shown to be a fast and low-material-consuming approach. These findings demonstrate that the UCT can be used to efficiently extract AMF spores in future research.
监测丛枝菌根真菌（AMF）孢子库的动态变化对于农业生态系统的可持续管理和保护至关重要。从土壤中提取AMF孢子最常用的方法是湿筛法（WST）。然而，这种方法有许多缺点。在本研究中，我们采用新方法对WST进行了改进：超声湿筛法（UWST）和超声离心法（UCT）。我们对从WST、UWST和UCT获得的孢子数量和质量进行了计数和比较，以验证新的改进技术。我们从土耳其凡湖盆地采集的不同植物根际中提取了AMF孢子，这些植物包括小麦（L.）、豆类（L.）、番茄（L.）、辣椒（L.）、欧芹（Mill.）和草坪草（L.）。在小麦和草坪草根际中观察到的AMF孢子数量最高和最低。UCT能从所有根际中提取出最多数量的孢子，其次是UWST和WST。UWST和WST能从小麦、辣椒、欧芹和草坪草根际中提取出相似数量的孢子。除了提取的孢子数量多之外，UCT还被证明是一种快速且耗材少的方法。这些发现表明，UCT可用于未来研究中高效提取AMF孢子。
