Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of Korea.
Department of Polar Sciences, University of Science and Technology, Incheon 21990, Republic of Korea.
Mar Drugs. 2023 Aug 17;21(8):454. doi: 10.3390/md21080454.
Polar microorganisms produce biologically active compounds that enable them to survive in harsh environments. These compounds have potential biomedical applications. The green microalga KNF0041, isolated from Arctic sea ice, has been found to produce polyunsaturated fatty acids (PUFAs), including omega-3 and omega-6, which have antioxidant properties. To improve the biomass production of strain KNF0041, statistical methods such as the Plackett-Burman design, Box-Behnken design, and response surface methodology (RSM) were utilized for medium optimization. The optimized medium was designed with increased potassium phosphate content and reduced acetic acid (AcOH) content. The use of the optimized medium resulted in an increase in the cell number as biomass of strain KNF0041 by 34.18% and the omega-3 and omega-6 fatty acid (FA) content by 10.04% and 58.29%, respectively, compared to that in normal TAP medium, which is known as the growth medium for culture. In this study, was discovered for the first time in the polar region and identified using morphology and molecular phylogenetic analyses, the secondary structures of the internal transcribed spacers, and optimized culture conditions. The results of this study provide an efficient method for the application of polar microalgae for the production of bioactive compounds.
极地微生物产生具有生物活性的化合物，使它们能够在恶劣的环境中生存。这些化合物具有潜在的生物医学应用。从北极海冰中分离出的绿微藻 KNF0041 被发现产生多不饱和脂肪酸 (PUFA)，包括具有抗氧化特性的 omega-3 和 omega-6。为了提高 KNF0041 菌株的生物量产量，使用 Plackett-Burman 设计、Box-Behnken 设计和响应面法 (RSM) 等统计方法对培养基进行了优化。优化后的培养基设计增加了磷酸钾的含量，减少了乙酸 (AcOH) 的含量。与已知的培养生长培养基 TAP 培养基相比，使用优化后的培养基使 KNF0041 菌株的细胞数量和 omega-3 和 omega-6 脂肪酸 (FA) 含量分别增加了 34.18%和 10.04%和 58.29%。在本研究中，首次在极地发现并通过形态学和分子系统发育分析、内部转录间隔区的二级结构和优化的培养条件进行了鉴定。本研究的结果为利用极地微藻生产生物活性化合物提供了一种有效的方法。
