Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 21990, South Korea.
Department of Polar Sciences, University of Science and Technology, Incheon, 21990, South Korea.
Appl Biochem Biotechnol. 2019 Jun;188(2):326-337. doi: 10.1007/s12010-018-2921-1. Epub 2018 Nov 15.
A lipid-producing microalga, Chlamydomonas sp. KNF0008, collected from the Arctic was capable of growing at temperatures ranging from 4 to 20 °C, and the highest cell density was measured at 15 °C and 100 μmol photons m s light intensity under continuous shaking and external aeration. KNF0008 showed the elevated accumulation of lipid bodies under nitrogen-deficient conditions, rather than under nitrogen-sufficient conditions. Fatty acid production of KNF0008 was 4.2-fold (104 mg L) higher than that of C. reinhardtii CC-125 at 15 °C in Bold's Basal Medium. The dominant fatty acids were C16:0, C16:4, C18:1, and C18:3, and unsaturated fatty acids (65.69%) were higher than saturated fatty acids (13.65%) at 15 °C. These results suggested that Arctic Chlamydomonas sp. KNF0008 could possibly be utilized for production of biodiesel during periods of cold weather because of its psychrophilic characteristics.
从北极采集的产脂微藻 Chlamydomonas sp. KNF0008 能够在 4 至 20°C 的温度范围内生长，在 15°C 和 100 μmol 光子 m s 连续摇床和外部通气下，光强最高的细胞密度为 15°C。在氮缺乏条件下，KNF0008 表现出脂滴的积累增加，而不是在氮充足条件下。KNF0008 在 15°C 时，在 Bold's Basal Medium 中的脂肪酸产量比 C. reinhardtii CC-125 高 4.2 倍（104mg L）。优势脂肪酸为 C16:0、C16:4、C18:1 和 C18:3，不饱和脂肪酸（65.69%）高于饱和脂肪酸（13.65%）在 15°C。这些结果表明，由于其耐寒特性，北极 Chlamydomonas sp. KNF0008 可能在寒冷天气期间用于生产生物柴油。
