Department of Regenerative Dental Medicine, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Department of Oral Health Care Promotion, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Immunopharmacol Immunotoxicol. 2024 Feb;46(1):49-54. doi: 10.1080/08923973.2023.2250551. Epub 2023 Aug 30.
Periodontitis is a chronic inflammatory disease induced by periodontal disease-causing bacteria. It has been shown that excessive immune response against bacteria is involved in periodontal tissue destruction including alveolar bone resorption. Erucin is a biologically active substance found in cruciferous plants such as arugula and is classified as an isothiocyanate. No previous studies have attempted to use erucin in the treatment of periodontitis, and there are no papers that have examined the effects of erucin on periodontal resident cells. The purpose of this study was to analyze the effects of erucin on the production of inflammatory and antioxidant mediators produced by tumor necrosis factor (TNF)-α-stimulated TR146 cells, an oral epithelial cell line, including its effects on signaling molecules.
Cytokine and chemokine levels were measured by ELISA. Protein expression in TR146 cells and activations of signal transduction pathway were determined by Western blotting.
Our results indicate that erucin suppresses interleukin-6 and CXC-chemokine ligand 10 production and vascular cell adhesion molecule-1 expression in TNF-α-stimulated TR146 cells. In addition, erucin induced the production of the antioxidant enzymes, Heme Oxygenase-1 and NAD(P)H quinone dehydrogenase 1 in TR146 cells. Furthermore, erucin suppressed TNF-α-stimulated nuclear factor-κB, signal transducer and activator of transcription3, and phospho-70S6 Kinase-S6 ribosomal protein signaling pathways in TR146 cells. We have shown that erucin has anti-inflammatory effects on oral epithelial cells and also induces the production of antioxidant mediators.
These results suggest that erucin may provide a new anti-inflammatory agent that can be used in the treatment of periodontitis.
牙周炎是一种由牙周病致病菌引起的慢性炎症性疾病。研究表明，过度的免疫反应对细菌参与牙周组织破坏，包括牙槽骨吸收。山萮菜酰胺是一种存在于芸薹属植物中的生物活性物质，属于异硫氰酸盐。以前没有研究尝试过使用山萮菜酰胺治疗牙周炎，也没有研究山萮菜酰胺对牙周常驻细胞影响的论文。本研究旨在分析山萮菜酰胺对肿瘤坏死因子（TNF）-α刺激的口腔上皮细胞系 TR146 细胞产生的炎症和抗氧化介质的产生的影响，包括对信号分子的影响。
通过 ELISA 测定细胞因子和趋化因子水平。通过 Western blot 测定 TR146 细胞中的蛋白表达和信号转导途径的激活。
我们的结果表明，山萮菜酰胺抑制 TNF-α刺激的 TR146 细胞中白细胞介素-6 和 CXC 趋化因子配体 10 的产生和血管细胞黏附分子-1 的表达。此外，山萮菜酰胺诱导 TR146 细胞中抗氧化酶血红素加氧酶-1 和 NAD（P）H 醌氧化还原酶 1 的产生。此外，山萮菜酰胺抑制 TNF-α刺激的 TR146 细胞中核因子-κB、信号转导和转录激活因子 3 和磷酸化 70S6 激酶-S6 核糖体蛋白信号通路。我们已经表明，山萮菜酰胺对口腔上皮细胞具有抗炎作用，并且还诱导抗氧化介质的产生。
这些结果表明，山萮菜酰胺可能为治疗牙周炎提供一种新的抗炎剂。
