Department of Regenerative Dental Medicine, Institute Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-Cho, Tokushima, Tokushima, 770-8504, Japan.
Department of Oral Health Care Promotion, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Tokushima, Japan.
Naunyn Schmiedebergs Arch Pharmacol. 2024 Apr;397(4):2233-2240. doi: 10.1007/s00210-023-02761-6. Epub 2023 Oct 7.
Berteroin is a bioactive substance classified as an isothiocyanate found in cruciferous vegetables such as cabbage, arugula, and salad leaves. In this study, we aimed to determine whether berteroin exerts anti-inflammatory effects on human periodontal ligament cells (HPDLCs), a resident cells of periodontal tissue. Berteroin suppressed interleukin (IL)-1β or tumor necrosis factor (TNF)-α-induced chemokines (C-C motif chemokine ligand (CCL)2, CCL20, C-X-C motif chemokine ligand (CXCL)10, IL-8, and IL-6) production and intercellular adhesion molecule (ICAM)-1 expression in HPDLCs. In addition, berteroin inhibited phosphorylation of IκB kinase (IKK)- α/ β, nuclear factor (NF)- κB p65, and IκB- α and degradation of IκB- α in the NF-κB pathway induced by IL-1 β or TNF- α stimulation. Moreover, berteroin could inhibit signal transducer and activator of transcription (STAT)3 phosphorylation in TNF- α -stimulated HPDLC. Furthermore, berteroin increased the expression of the antioxidant enzymes, heme oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase (NQO)1, in IL-1 β or TNF- α -stimulated HPDLCs. These results suggest that berteroin may decrease the production of inflammatory mediators in HPDLCs by suppressing the NF-κB pathway, and may also decrease the local reactive oxygen species (ROS) production in periodontal lesions by increasing the production of antioxidant enzymes.
伯替醇是一种生物活性物质，属于异硫氰酸盐，存在于十字花科蔬菜中，如卷心菜、芝麻菜和沙拉叶。在这项研究中，我们旨在确定伯替醇是否对人牙周韧带细胞（HPDLCs）发挥抗炎作用，HPDLCs 是牙周组织的常驻细胞。伯替醇抑制白细胞介素（IL）-1β 或肿瘤坏死因子（TNF）-α 诱导的趋化因子（C-C 基序趋化因子配体（CCL）2、CCL20、C-X-C 基序趋化因子配体（CXCL）10、IL-8 和 IL-6）的产生和细胞间黏附分子（ICAM）-1 在 HPDLCs 中的表达。此外，伯替醇抑制 IκB 激酶（IKK）-α/β、核因子（NF）-κB p65 和 IκB-α 在 IL-1β 或 TNF-α 刺激诱导的 NF-κB 通路中的磷酸化和 IκB-α 的降解。此外，伯替醇可抑制 TNF-α 刺激的 HPDLC 中信号转导和转录激活因子（STAT）3 的磷酸化。此外，伯替醇增加了抗氧化酶血红素加氧酶（HO）-1 和 NAD（P）H 醌氧化还原酶（NQO）1 在 IL-1β 或 TNF-α 刺激的 HPDLCs 中的表达。这些结果表明，伯替醇可能通过抑制 NF-κB 通路减少 HPDLCs 中炎症介质的产生，并通过增加抗氧化酶的产生减少牙周病变中局部活性氧（ROS）的产生。
