Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden.
Sci Adv. 2023 Aug 25;9(34):eadg1610. doi: 10.1126/sciadv.adg1610.
The next steps of deep space exploration are manned missions to Moon and Mars. For safe space missions for crew members, it is important to understand the impact of space flight on the immune system. We studied the effects of 21 days dry immersion (DI) exposure on the transcriptomes of T cells isolated from blood samples of eight healthy volunteers. Samples were collected 7 days before DI, at day 7, 14, and 21 during DI, and 7 days after DI. RNA sequencing of CD3 T cells revealed transcriptional alterations across all time points, with most changes occurring 14 days after DI exposure. At day 21, T cells showed evidence of adaptation with a transcriptional profile resembling that of 7 days before DI. At 7 days after DI, T cells again changed their transcriptional profile. These data suggest that T cells adapt by rewiring their transcriptomes in response to simulated weightlessness and that remodeling cues persist when reexposed to normal gravity.
下一步深空探索是载人登月和火星任务。为了确保机组人员太空任务的安全，了解太空飞行对免疫系统的影响很重要。我们研究了 21 天干式浸水（DI）暴露对从八名健康志愿者血液样本中分离的 T 细胞转录组的影响。样本在 DI 前 7 天、DI 第 7 天、14 天和 21 天以及 DI 后 7 天采集。对 CD3 T 细胞进行 RNA 测序显示，所有时间点都发生了转录变化，大多数变化发生在 DI 暴露后 14 天。在第 21 天，T 细胞表现出适应的迹象，其转录谱类似于 DI 前 7 天的转录谱。在 DI 后 7 天，T 细胞再次改变了它们的转录谱。这些数据表明，T 细胞通过重编转录组来适应模拟的失重，并且当重新暴露于正常重力时，重塑线索仍然存在。
