Kurup V P, Resnick A, Rose H D
Mycopathologia. 1986 Jul;95(1):25-8. doi: 10.1007/BF00436318.
Cottonseed protein agar and a modified Tween-albumin casein hydrolysate (TAC) medium were compared for the yeast phase conversion of Blastomyces dermatitidis strains including fresh isolates as well as strains maintained in long-term storage. It was found that both media converted all the B. dermatitidis (mycelial phase) strains studied to yeast phase in three days. The TAC medium has the added advantage that it is clear and the growth can be recognized earlier than in the opaque cottonseed agar medium. The conversion in most cases was more than 95% and the morphology of the yeast cells was uniformly typical with broad base budding. There was a striking difference between the sensitivity of the yeast and mycelial phases of B. dermatitidis strains. The yeast phase was usually more sensitive to Amphotericin B than the mycelial phase of B. dermatitidis. Similarly, the yeast phases of four out of six strains were more sensitive to ketoconazole than their respective mycelial phases, while two strains showed identical sensitivity in cottonseed agar. The yeast phase organism was more susceptible to Amphotericin B when cottonseed medium was used whereas the yeast phase showed more susceptibility to ketoconazole in TAC medium. Since the sensitivity among the various strains differed, it is necessary to determine the antifungal susceptibility of the pathogenic phase of the organism for initiating proper therapy and monitoring effectiveness.
对棉籽蛋白琼脂和改良吐温 - 白蛋白酪蛋白水解物（TAC）培养基进行了比较，以研究皮炎芽生菌菌株（包括新鲜分离株以及长期保存的菌株）的酵母相转化情况。结果发现，两种培养基均可在三天内将所有研究的皮炎芽生菌（菌丝相）菌株转化为酵母相。TAC培养基具有额外的优势，即它是澄清的，并且比不透明的棉籽琼脂培养基更早识别出其生长情况。在大多数情况下，转化率超过95%，酵母细胞的形态一致典型，呈宽基芽生。皮炎芽生菌菌株的酵母相和菌丝相的敏感性存在显著差异。皮炎芽生菌的酵母相通常比菌丝相对两性霉素B更敏感。同样，六株菌株中有四株的酵母相比各自的菌丝相对酮康唑更敏感，而在棉籽琼脂中两株菌株表现出相同的敏感性。当使用棉籽培养基时，酵母相生物体对两性霉素B更敏感，而在TAC培养基中酵母相对酮康唑更敏感。由于不同菌株之间的敏感性不同，有必要确定该生物体致病相的抗真菌药敏情况，以便开始适当的治疗并监测疗效。
