通过传统的BacT/ALERT微生物检测系统培养基成功复苏两例双相致病真菌。

Two cases of recovery of dimorphic pathogenic fungi via conventional BacT/ALERT microbial detection system media.

作者信息

Munson Erik, Endes Tina, Vaughan Katharine, Block Timothy, Hryciuk Jeanne E

机构信息

Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, WI 53226, USA.

出版信息

Mycopathologia. 2009 Apr;167(4):191-5. doi: 10.1007/s11046-008-9168-2. Epub 2008 Nov 18.

DOI:10.1007/s11046-008-9168-2

PMID:19015951

Abstract

We hereby report two instances of dimorphic fungus cultivation in BacT/ALERT-based bacteriologic media, with the first such characterization of Blastomyces dermatitidis. From a patient with disseminated coccidioidomycosis, routine blood cultures incubated on the MB/BacT 3D Microbial Detection System generated a positive signal following 75 h of incubation. B. dermatitidis was isolated from a patient hospitalized with a four-week course of respiratory illness. Organism detection from respiratory specimens via the MB/BacT 3D Mycobacteria Detection System occurred 5 days sooner than the routine fungus culture. Etiologic agents of endemic mycoses may be isolated in bacteriologic media employed by continuous monitoring instrumentation.

摘要

我们在此报告两例在基于BacT/ALERT的细菌学培养基中培养双相真菌的情况，其中首次对皮炎芽生菌进行了此类鉴定。一名播散性球孢子菌病患者的血液样本在MB/BacT 3D微生物检测系统上进行常规血培养，培养75小时后产生阳性信号。皮炎芽生菌分离自一名因呼吸道疾病住院四周的患者。通过MB/BacT 3D分枝杆菌检测系统从呼吸道标本中检测到该病原体比常规真菌培养早5天。地方性真菌病的病原体可能在连续监测仪器所用的细菌学培养基中分离出来。