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人脐带间充质干细胞片移植治疗小鼠心肌梗死的疗效

The Efficacy of Transplanting Human Umbilical Cord Mesenchymal Stem Cell Sheets in the Treatment of Myocardial Infarction in Mice.

作者信息

Bui Thang Quoc, Binh Nguyen Trong, Pham Truc Le-Buu, Le Van Trinh, Truong Nhung Hai, Nguyen Dang Phu-Hai, Luu Thao Thi-Thu, Nguyen-Xuan Pham Trang, Cam Tran Tu, Nguyen Huyen Thuong-Thi, Thuy-Trinh Nhu, Tran Phong Anh

机构信息

Cho Ray Hospital, Ho Chi Minh City 700000, Vietnam.

Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.

出版信息

Biomedicines. 2023 Aug 3;11(8):2187. doi: 10.3390/biomedicines11082187.

DOI:10.3390/biomedicines11082187
PMID:37626684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10452263/
Abstract

The transplantation of mesenchymal stem cell (MSC) sheets derived from human umbilical cords (hUCs) was investigated in this study as a potential application in treating myocardial infarction (MI). Two groups of hUC-MSC sheets were formed by populating LunaGel, which are 3D scaffolds of photo-crosslinkable gelatin-based hydrogel with two different cell densities. An MI model was created by ligating the left anterior descending coronary artery of healthy BALB/c mice. After two weeks, the cell sheets were applied directly to the MI area and the efficacy of the treatment was evaluated over the next two weeks by monitoring the mice's weight, evaluating the left ventricle ejection fraction, and assessing the histology of the heart tissue at the end of the experiment. Higher cell density showed significantly greater efficiency in MI mice treatment in terms of weight gain and the recovery of ejection fraction. The heart tissue of the groups receiving cell sheets showed human-CD44-positive staining and reduced fibrosis and apoptosis. In conclusion, the hUC-MSC sheets ameliorated heart MI injury in mice and the efficacy of the cell sheets improved as the number of cells increased.

摘要

本研究对源自人脐带（hUC）的间充质干细胞（MSC）片移植作为治疗心肌梗死（MI）的潜在应用进行了研究。通过接种LunaGel形成两组hUC-MSC片，LunaGel是具有两种不同细胞密度的基于光交联明胶的水凝胶的3D支架。通过结扎健康BALB/c小鼠的左冠状动脉前降支建立MI模型。两周后，将细胞片直接应用于MI区域，并在接下来的两周内通过监测小鼠体重、评估左心室射血分数以及在实验结束时评估心脏组织的组织学来评估治疗效果。就体重增加和射血分数的恢复而言，较高的细胞密度在MI小鼠治疗中显示出显著更高的效率。接受细胞片的组的心脏组织显示人CD44阳性染色，纤维化和细胞凋亡减少。总之，hUC-MSC片改善了小鼠的心脏MI损伤，并且随着细胞数量的增加，细胞片的疗效提高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/d15ed551a774/biomedicines-11-02187-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/271930c9b6f9/biomedicines-11-02187-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/1e71578f1aef/biomedicines-11-02187-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/14a7c5665ded/biomedicines-11-02187-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/5ab90520d817/biomedicines-11-02187-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/4fe951689603/biomedicines-11-02187-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/d15ed551a774/biomedicines-11-02187-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/271930c9b6f9/biomedicines-11-02187-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/1e71578f1aef/biomedicines-11-02187-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/14a7c5665ded/biomedicines-11-02187-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/5ab90520d817/biomedicines-11-02187-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/4fe951689603/biomedicines-11-02187-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630c/10452263/d15ed551a774/biomedicines-11-02187-g006.jpg

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Short lifespan of syngeneic transplanted MSC is a consequence of in vivo apoptosis and immune cell recruitment in mice.同种异体移植 MSC 寿命短是体内细胞凋亡和免疫细胞募集导致的。
Cell Death Dis. 2021 Jun 2;12(6):566. doi: 10.1038/s41419-021-03839-w.
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Cell sheet formation enhances the therapeutic effects of human umbilical cord mesenchymal stem cells on myocardial infarction as a bioactive material.
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Bioact Mater. 2021 Mar 5;6(9):2999-3012. doi: 10.1016/j.bioactmat.2021.01.036. eCollection 2021 Sep.
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