Kim Chae Jeong, Jang Daegyu, Lim Do-Hwan
School of Systems Biomedical Science, Soongsil University, Seoul, Republic of Korea.
Anim Cells Syst (Seoul). 2024 Dec 24;29(1):35-45. doi: 10.1080/19768354.2024.2444366. eCollection 2025.
Tissue growth is controlled by various signaling pathways, such as the insulin/IGF-signaling (IIS) pathway. Although IIS activation is regulated by a complex regulatory network, the mechanism underlying miRNA-based regulation of the IIS pathway in wing development remains unclear. In this study, we found that the wing size of adult flies was negatively affected by miR-263b expression. The miR-263b-mediated alteration in wing size was linked to a reduction in wing cell number. Additionally, overexpression in S2 cells decreased cell proliferation and increased cell death. Consequently, we identified as a direct target of miR-263b-5p and found that miR-263b-mediated wing growth regulation was due to changes in expression. Co-expression of in -overexpressing wings rescued the overexpression-mediated reduction in wing growth. These results enhance our understanding of the crucial role of miRNAs in growth regulation during wing development.
组织生长受多种信号通路控制，如胰岛素/胰岛素样生长因子信号（IIS）通路。尽管IIS激活受复杂调控网络调节，但在翅膀发育过程中基于miRNA对IIS通路的调控机制仍不清楚。在本研究中，我们发现成年果蝇的翅膀大小受到miR-263b表达的负面影响。miR-263b介导的翅膀大小改变与翅膀细胞数量减少有关。此外，在S2细胞中过表达会降低细胞增殖并增加细胞死亡。因此，我们鉴定出[具体基因名称]为miR-263b-5p的直接靶标，并发现miR-263b介导的翅膀生长调控是由于[具体基因名称]表达的变化。在[过表达miR-263b的果蝇品系名称]过表达翅膀中共同表达[具体基因名称]挽救了[miR-263b]过表达介导的翅膀生长减少。这些结果增强了我们对miRNA在翅膀发育过程中生长调控的关键作用的理解。
