Patangia Dhrati V, Grimaud Ghjuvan, Linehan Kevin, Ross R Paul, Stanton Catherine
School of Microbiology, University College Cork, T12 K8AF Cork, Ireland.
Biosciences Building, Teagasc Food Research Centre, P61 C996 Fermoy, Ireland.
Antibiotics (Basel). 2023 Aug 14;12(8):1315. doi: 10.3390/antibiotics12081315.
This study investigated the longitudinal impact of methods for the drying off of cows with and without dry cow therapy (DCT) on the microbiota and resistome profile in colostrum and milk samples from cows. Three groups of healthy dairy cows ( = 24) with different antibiotic treatments during DCT were studied. Colostrum and milk samples from Month 0 (M0), 2 (M2), 4 (M4) and 6 (M6) were analysed using whole-genome shotgun-sequencing. The microbial diversity from antibiotic-treated groups was different and higher than that of the non-antibiotic group. This difference was more evident in milk compared to colostrum, with increasing diversity seen only in antibiotic-treated groups. The microbiome of antibiotic-treated groups clustered separately from the non-antibiotic group at M2-, M4- and M6 milk samples, showing the effect of antibiotic treatment on between-group (beta) diversity. The non-antibiotic group did not show a high relative abundance of mastitis-causing pathogens during early lactation and was more associated with genera such as , , and . A high relative abundance of antibiotic resistance genes (ARGs) was observed in the milk of antibiotic-treated groups with the Cephaguard group showing a significantly high abundance of genes conferring resistance to cephalosporin, aminoglycoside and penam classes. The data support the use of non-antibiotic alternatives for drying off in cows.
本研究调查了采用和不采用干奶牛疗法（DCT）对奶牛进行干奶处理的方法，对奶牛初乳和牛奶样本中微生物群和耐药基因组概况的纵向影响。研究了三组在干奶期接受不同抗生素处理的健康奶牛（ = 24）。使用全基因组鸟枪法测序分析了第0个月（M0）、第2个月（M2）、第4个月（M4）和第6个月（M6）的初乳和牛奶样本。抗生素处理组的微生物多样性有所不同，且高于非抗生素组。与初乳相比，这种差异在牛奶中更为明显，仅在抗生素处理组中观察到多样性增加。在M2、M4和M6的牛奶样本中，抗生素处理组的微生物群与非抗生素组分开聚类，显示出抗生素处理对组间（β）多样性的影响。非抗生素组在泌乳早期未显示出引起乳腺炎的病原体的高相对丰度，且更多地与诸如、、和等属相关。在抗生素处理组的牛奶中观察到抗生素抗性基因（ARG）的高相对丰度，其中头孢菌素保护组显示出对头孢菌素、氨基糖苷类和青霉烯类具有抗性的基因的显著高丰度。数据支持在奶牛干奶时使用非抗生素替代品。
