Somers G, Blondel B, Orci L, Malaisse W J
Endocrinology. 1979 Jan;104(1):255-64. doi: 10.1210/endo-104-1-255.
Monolayer cultures of neonatal rat endocrine pancreatic cells were examined by time lapse cinematography. Motion analysis revealed two major types of motile events. On one hand, particles (0.3 micrometers in diameter) tentatively identified as secretory granules were found to undergo back and forth saltatory movements along oriented pathways. The existence of such pathways was statistically validated by the large prevalence of angles close to either 0 degrees of 180 degrees between successive movements of the same particle. The movements occurred at a mean speed of 0.8 micrometers/sec. Glucose (16.7 mM) increased the frequency of such movements. Vincristine (10 microM) caused a progressive inhibition of saltatory movements, which may depend, therefore, on the integrity of the microtubular apparatus. On the other hand, areas of the cell boundary displayed contractile-like movements, which were stimulated by insulinotropic agents, such as glucose and the ionophore A23187. Cytochalasin B also affected this second type of motile event, which is thought to reflect the activity of actin-like microfilaments. These findings suggest that the microtubular apparatus serves as a guiding cytoskeleton for the oriented translocation of secretory granules, whereas the microfilamentous cell web may control the eventual access of the granules to exocytotic sites.
通过延时摄影术对新生大鼠内分泌胰腺细胞的单层培养物进行了检查。运动分析揭示了两种主要的运动事件类型。一方面，初步鉴定为分泌颗粒的颗粒（直径0.3微米）被发现沿着定向路径进行来回跳跃运动。同一颗粒连续运动之间接近0度或180度的角度大量存在，从统计学上验证了这种路径的存在。这些运动的平均速度为0.8微米/秒。葡萄糖（16.7 mM）增加了此类运动的频率。长春新碱（10 microM）导致跳跃运动逐渐受到抑制，因此，这可能取决于微管装置的完整性。另一方面，细胞边界区域表现出类似收缩的运动，这些运动受到促胰岛素剂的刺激，如葡萄糖和离子载体A23187。细胞松弛素B也影响了这第二种运动事件类型，据认为这反映了肌动蛋白样微丝的活性。这些发现表明，微管装置作为分泌颗粒定向转运的引导性细胞骨架，而微丝细胞网络可能控制颗粒最终到达胞吐位点。
