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[胎牛胰岛细胞的显微电影摄影研究]

[Microcinematographic study of pancreatic islet cells from fetal cattle].

作者信息

Bliumkin V N, Babikova R A, Kokorin I N

出版信息

Biull Eksp Biol Med. 1978 Feb;85(2):202-5.

PMID:343834
Abstract

The growth characteristics of pancreatic islet cell cultures of bovine fetuses were investigated by the method of time-lapse cinemicrography. These monolayer cultures consisted of epithelial cells only. Under the influenc of high glucose concentration in the growth medium (up to 300 mg per 100 ml) there occurred an activation of mitochondrial apparatus of the islet cells, stimulation of cytogranulokinesis, and intensification of accumulation of the secretory granules with the subsequent degranulation of the islet cells. These data were compared with the results of cytological control of fixed and stained culture.

摘要

采用延时显微电影摄影术研究了牛胎儿胰岛细胞培养物的生长特性。这些单层培养物仅由上皮细胞组成。在生长培养基中高葡萄糖浓度(高达每100毫升300毫克)的影响下,胰岛细胞的线粒体装置被激活,细胞颗粒分裂受到刺激,分泌颗粒的积累增强,随后胰岛细胞脱颗粒。将这些数据与固定和染色培养物的细胞学对照结果进行了比较。

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