Henan International Laboratory for Green Pest Control, Henan Engineering Laboratory of Pest Biological Control, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Genes (Basel). 2023 Jul 26;14(8):1528. doi: 10.3390/genes14081528.
Ants are the standout group among eusocial insects in terms of their exceptional species richness and ecological dominance. The phylogenetic relationships among the group remain elusive. Mitochondrial genome sequences, as a kind of molecular marker, have been widely utilized in the phylogenetic analysis of insects. However, the number of ant mitogenomes published is still very limited. In this study, we utilized next-generation sequencing to determine the complete mitogenome of (Formicidae, Ponerinae). This is the first mitogenome from the genus . Two gene rearrangements were identified in the mitogenome, the transposition of and and the transposition of and . The secondary structures of tRNAs were predicted. The tRNA genes and lacked the dihydrouridine (DHU) arm, and the lacked the TΨC (T) arm. Phylogenetic analyses of the mitochondrial protein-coding genes under maximum likelihood (ML) and Bayesian inference (BI) criteria resulted in conflicting hypotheses. BI analysis using amino acid data with the site-heterogeneous mixture model produced a tree topology congruent with previous studies. The Formicidae was subdivided into two main clades, namely the "poneroid" clade and the "formicoid" clade. A sister group relationship between Myrmicinae and Formicinae was recovered within the "formicoid" clade.
蚂蚁在社会性昆虫中是物种丰富度和生态优势最为突出的类群。该类群的系统发育关系仍难以捉摸。线粒体基因组序列作为一种分子标记，已广泛应用于昆虫的系统发育分析。然而，已发表的蚂蚁线粒体基因组数量仍然非常有限。本研究利用新一代测序技术确定了（膜翅目，蚁科，细猛蚁亚科）的完整线粒体基因组。这是该属的第一个线粒体基因组。在该线粒体基因组中发现了两个基因重排，即 和 的转位以及 和 的转位。预测了 tRNA 的二级结构。tRNA 基因 和 缺乏二氢尿嘧啶 (DHU)臂，而 缺乏 TΨC (T)臂。基于最大似然法 (ML)和贝叶斯推断法 (BI)的线粒体蛋白编码基因的系统发育分析产生了相互矛盾的假说。使用氨基酸数据和位点异质混合模型的 BI 分析产生的树拓扑结构与先前的研究一致。蚁科分为两个主要分支，即“猛蚁分支”和“蚁分支”。在“蚁分支”内恢复了蚁亚科和细猛蚁亚科的姊妹群关系。
