Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Jeonju 54874, Republic of Korea.
Department of Agricultural Engineering, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Jeonju 54875, Republic of Korea.
Genes (Basel). 2023 Aug 6;14(8):1593. doi: 10.3390/genes14081593.
Tiller number is an important trait associated with yield in rice. Tiller number in Korean rice was analyzed under greenhouse conditions in 160 recombinant inbred lines (RILs) derived from a cross between the temperate varieties Odae and Unbong40 to identify quantitative trait loci (QTLs). A genetic map comprising 239 kompetitive allele-specific PCR (KASP) and 57 cleaved amplified polymorphic sequence markers was constructed. , a major QTL for tiller number, was identified at 132.4 cm on chromosome 3. This QTL was also detected under field conditions in a backcross population; thus, was stable across generations and environments. co-located with QTLs associated with panicle number per plant and culm diameter, indicating it had pleiotropic effects. The regions of Odae and Unbong40 differed in a known functional variant (4 bp TGTG insertion/deletion) in the 5' UTR of , a gene underlying variation in tiller number and culm strength. Investigation of variation in genotype and tiller number revealed that varieties with the insertion genotype had lower tiller numbers than those with the reference genotype. A high-resolution melting marker was developed to enable efficient marker-assisted selection. The QTL will therefore be useful in breeding programs developing varieties with optimal tiller numbers for increased yield.
分蘖数是与水稻产量相关的一个重要性状。在温室条件下，对来源于温带品种 Odae 和 Unbong40 的杂交后代 160 个重组自交系（RIL）的韩国水稻分蘖数进行了分析，以鉴定数量性状位点（QTL）。构建了一张包含 239 个竞争性等位基因特异性 PCR（KASP）和 57 个切割扩增多态性序列标记的遗传图谱。在第 3 染色体上 132.4cm 处鉴定到一个控制分蘖数的主效 QTL，该 QTL 在回交群体的田间条件下也被检测到；因此，该 QTL 在不同世代和环境下是稳定的。与单株穗数和茎直径相关的 QTL 与 共定位，表明其具有多效性。Odae 和 Unbong40 的 区域在一个已知的功能变体（5'UTR 中的 4bp TGTG 插入/缺失）中存在差异，该变体是控制分蘖数和茎强度变异的基因。对基因型和分蘖数的变异进行调查表明，具有插入基因型的品种的分蘖数低于参考基因型的品种。开发了一个高分辨率熔解标记，以实现高效的标记辅助选择。因此，该 QTL 将有助于开发具有最佳分蘖数以提高产量的品种的育种计划。
