Sawyer H R, Abel J H, McClellan M C, Schmitz M, Niswender G D
Endocrinology. 1979 Feb;104(2):476-86. doi: 10.1210/endo-104-2-476.
To study the relationship between formation and release of Golgi-derived secretory granules and progesterone secretion, slices of ovine luteal tissue were incubated in the presence of LH and/or calcium ionophore A23187. Increases in progesterone secretion in response to LH and/or ionophore were accompanied by a concomitant release of secretory granules. In contrast, in the presence of colchicine, LH-stimulated progesterone secretion was significantly reduced (P less than 0.01), granule formation appeared to be blocked, and there was little evidence of exocytosis. In addition, unusual pleomorphic membrane-bounded saccules containing an electron-dense material were abundant throughout the centrospheric region of cells treated with colchicine. Because of the close parallelism between formation and release of Golgi-derived secretory granules and progesterone secretion, it appears that progesterone secretion may be coupled to exocytosis of secretory granules. Although the exact content and function of the secretory granules described remains to be elucidated, the data obtained are compatible with the notion that they may contain a progesterone carrier protein.
为了研究高尔基体衍生的分泌颗粒的形成与释放和孕酮分泌之间的关系，将绵羊黄体组织切片在促黄体生成素（LH）和/或钙离子载体A23187存在的情况下进行孵育。对LH和/或离子载体的反应中孕酮分泌的增加伴随着分泌颗粒的同步释放。相反，在秋水仙碱存在的情况下，LH刺激的孕酮分泌显著减少（P<0.01），颗粒形成似乎被阻断，并且几乎没有胞吐作用的证据。此外，在用秋水仙碱处理的细胞的中心球区域中，含有电子致密物质的异常多形性膜结合囊泡大量存在。由于高尔基体衍生的分泌颗粒的形成与释放和孕酮分泌之间存在密切的平行关系，似乎孕酮分泌可能与分泌颗粒的胞吐作用相关联。尽管所描述的分泌颗粒的确切内容和功能仍有待阐明，但所获得的数据与它们可能含有孕酮载体蛋白的观点相符。
