Higuchi T, Kaneko A, Abel J H, Niswender G D
Endocrinology. 1976 Oct;99(4):1023-32. doi: 10.1210/endo-99-4-1023.
When slices of ovine luteal tissue were perfused with medium containing luteinizing hormone (LH), the output of progesterone was increased significantly (P less than 0.01) in eleven of twelve experiments. However, addition of LH to the medium did not influence the luteal cell membrane potential. The addition of 47 mM potassium to the medium resulted in increased progesterone output (P less than 0.01) and depolarization of the luteal cell membrane within 2 min. Progesterone output decreased to approximate pretreatment levels within 2 min of the return to normal potassium levels in the perfusion medium. High levels of potassium further increased the output of progesterone from tissue stimulated with LH. Perfusion of the slices with sodium-free medium also resulted in increased (P less than 0.01) progesterone output within 2 min, which returned to pretreatment levels within 2 min after normal sodium levels were restored to the medium. Perfusion of the slices with sodium-free medium did not influence the membrane potential. Perfusion of the tissue with LH, 47 mM potassium, or sodium-free medium had no effect on progesterone output if the medium was calcium-free and/or contained 2 mM EGTA. These data suggested that the calcium ion plays an important role in mediating the steroidogenic response of ovine luteal tissue to LH. A second series of experiments was designed to ascertain if luteal cells were coupled electrically. Sixty-six pairs of luteal cells separated by 150-300 mum were penetrated with electrodes and the membrane potential of both cells was studied. One cell of each pair was hyperpolarized by passage of 0.4 nA current into the cell, but in no case was there an effect on the membrane potential of the other penetrated cell. Likewise, when five cells were injected iontophoretically with Procion Yellow there was no evidence of diffusion of the dye to adjacent cells. There was no evidence obtained in this study which suggested that ovine luteal cells were coupled electrically.
当用含有促黄体生成素（LH）的培养基灌注绵羊黄体组织切片时，在12次实验中的11次，孕酮的分泌量显著增加（P<0.01）。然而，向培养基中添加LH并不影响黄体细胞膜电位。向培养基中添加47 mM钾会导致孕酮分泌量增加（P<0.01），并在2分钟内使黄体细胞膜去极化。在灌注培养基恢复到正常钾水平后的2分钟内，孕酮分泌量降至接近预处理水平。高水平的钾进一步增加了LH刺激的组织中孕酮的分泌量。用无钠培养基灌注切片也会在2分钟内导致孕酮分泌量增加（P<0.01），在培养基恢复到正常钠水平后的2分钟内又恢复到预处理水平。用无钠培养基灌注切片不影响膜电位。如果培养基无钙和/或含有2 mM乙二醇双四乙酸（EGTA），用LH、47 mM钾或无钠培养基灌注组织对孕酮分泌量没有影响。这些数据表明钙离子在介导绵羊黄体组织对LH的类固醇生成反应中起重要作用。设计了第二系列实验来确定黄体细胞是否电偶联。用微电极刺入66对相距150 - 300μm的黄体细胞，并研究两个细胞的膜电位。每对细胞中的一个细胞通过通入0.4 nA电流而发生超极化，但在任何情况下对另一个刺入细胞的膜电位都没有影响。同样，当对五个细胞进行离子电泳注射普施安黄时，没有证据表明染料扩散到相邻细胞。在本研究中没有获得证据表明绵羊黄体细胞是电偶联的。
