National Citrus Improvement Center, Hunan Agricultural University (Changsha Branch), Changsha 410128, China.
College of Horticulture, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2023 Aug 10;24(16):12644. doi: 10.3390/ijms241612644.
The ETHYLENE INSENSITIVE3-LIKE (EIL) family is one of the most important transcription factor (TF) families in plants and is involved in diverse plant physiological and biochemical processes. In this study, ten EIL transcription factors (CsEILs) in sweet orange were systematically characterized via whole-genome analysis. The genes were unevenly distributed across the four sweet orange chromosomes. Putative -acting regulatory elements (CREs) associated with were found to be involved in plant development, as well as responses to biotic and abiotic stress. Notably, quantitative reverse transcription polymerase chain reaction (qRT-PCR) revealed that genes were widely expressed in different organs of sweet orange and responded to both high and low temperature, NaCl treatment, and to ethylene-dependent induction of transcription, while eight additionally responded to pv. (Xcc) infection, which causes citrus canker. Among these, , and showed pronounced upregulation. Moreover, nine genes exhibited differential expression in response to Liberibacter asiaticus (Las) infection, which causes Citrus Huanglongbing (HLB). The genome-wide characterization and expression profile analysis of genes provide insights into the potential functions of the family in disease resistance.
ETHYLENE INSENSITIVE3-LIKE（EIL）家族是植物中最重要的转录因子（TF）家族之一，参与多种植物生理和生化过程。本研究通过全基因组分析对甜橙中的十个 EIL 转录因子（CsEILs）进行了系统表征。这些基因在甜橙的四条染色体上不均匀分布。与 相关的假定 - 作用调节元件（CREs）被发现参与植物发育以及对生物和非生物胁迫的反应。值得注意的是，定量逆转录聚合酶链反应（qRT-PCR）显示，基因在甜橙不同器官中广泛表达，并对高温和低温、NaCl 处理以及乙烯依赖性转录诱导有反应，而另外八个基因对 pv. （Xcc）感染有反应，导致柑橘溃疡病。其中， 、 和 表现出明显的上调。此外，九个基因在响应引起柑橘黄龙病（HLB）的 Liberibacter asiaticus（Las）感染时表现出差异表达。基因的全基因组特征和表达谱分析为该家族在抗病性中的潜在功能提供了深入了解。
