State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, China.
Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N OX2, Canada.
Genes (Basel). 2022 Jul 22;13(8):1299. doi: 10.3390/genes13081299.
infection leads to hypertrophy of host roots and subsequent formation of galls, causing huge economic losses to agricultural producers of plants. Ethylene (ET) has been reported to play a vital role against necrotrophic pathogens in the classic immunity system. More clues suggested that the defense to pathogens in roots may be different from the acrial. The ET pathway may play a positive role in the infection of . , as shown by recent transcriptome profiling. However, the molecular basis of ET remains poorly understood. In this study, we investigated the potential role of ethylene against . infection in an double-mutant of (). After infection, (Disease Index/DI: 93) showed more susceptibility compared with wild type (DI: 75). Then, we inoculated () with . by 1-aminocyclopropane-1-carboxylic acid (ACC) and pyrazinamide (PZA), respectively. It was found that the symptoms of infected roots with ACC were more serious than those with PZA at 20 dpi (day post infection). However, the DI were almost the same in different treatments at 30 dpi. can be directly regulated by ET and was upregulated at 7 dpi with ACC, as shown by qRT-PCR. The mutant of (DI: 93.75) was more susceptible than the wild type in . Thus, our work reveals the dual roles of ET in infection of and provides evidence of ET in root defense against pathogens.
感染导致宿主根的肥大和随后的瘿形成，给植物的农业生产者造成巨大的经济损失。已报道乙烯 (ET) 在经典免疫系统中对坏死性病原体起着至关重要的作用。更多的线索表明，根部对病原体的防御可能与地上部分不同。ET 途径可能在感染中发挥积极作用，最近的转录组分析表明。然而，ET 的分子基础仍知之甚少。在这项研究中，我们研究了乙烯在 （）双突变体中的潜在作用。感染后，（疾病指数/DI：93）比野生型（DI：75）更易感染。然后，我们用 1-氨基环丙烷-1-羧酸（ACC）和吡嗪酰胺（PZA）分别接种 （）。结果发现，感染根的症状用 ACC 接种比用 PZA 接种在 20 dpi 时更严重。然而，在 30 dpi 时，不同处理之间的 DI 几乎相同。可以被 ET 直接调控，如 qRT-PCR 所示，在 7 dpi 时，ACC 上调。与野生型相比，（DI：93.75）突变体在 中更易感染。因此，我们的工作揭示了 ET 在感染中的双重作用，并为 ET 在根防御病原体方面提供了证据。
