Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Int J Mol Sci. 2023 Aug 11;24(16):12666. doi: 10.3390/ijms241612666.
-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-associated diseases. In this present study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and RNA-seq analyses were conducted to comprehensively analyze the in vitro anti-inflammatory effects of 10-HDA on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Our results demonstrated that 128 differentially expressed metabolites and 1721 differentially expressed genes were identified in the 10-HDA-treated groups compared to the LPS groups. Metabolites were significantly enriched in amino acid metabolism pathways, including methionine metabolism, glycine and serine metabolism, and tryptophan metabolism. The differentially expressed genes enrichment analysis indicated that antigen processing and presentation, NOD-like receptor signaling pathway, and arginine biosynthesis were enriched with the administration of 10-had. The correlation analysis revealed that glycerophospholipid metabolism and s-adenosylmethionine-dependent methylation processes might be involved in the response to the 10-HDA treatment. Overall, the findings from this study showed that 10-HDA might involve the modulation of certain signaling pathways involved in the inflammatory response, but further research is needed to determine the safety and efficacy as a therapeutic agent.
10-羟基-2-癸烯酸（10-HDA）是蜂王浆中特有的一种脂肪酸，具有潜在的健康益处，如抗炎作用。然而，为了充分了解其在炎症相关疾病中的作用机制和治疗潜力，还需要进一步研究。在本研究中，采用液相色谱-串联质谱（LC-MS/MS）和 RNA-seq 分析方法，全面分析了 10-HDA 对脂多糖（LPS）刺激的 RAW 264.7 细胞的体外抗炎作用。结果表明，与 LPS 组相比，10-HDA 处理组有 128 个差异表达代谢物和 1721 个差异表达基因。代谢物在氨基酸代谢途径中显著富集，包括蛋氨酸代谢、甘氨酸和丝氨酸代谢以及色氨酸代谢。差异表达基因富集分析表明，10-HDA 给药后抗原加工和呈递、NOD 样受体信号通路和精氨酸生物合成途径富集。相关性分析表明，甘油磷脂代谢和 s-腺苷甲硫氨酸依赖性甲基化过程可能参与了对 10-HDA 处理的反应。总的来说，这项研究的结果表明，10-HDA 可能参与了调节某些与炎症反应相关的信号通路，但还需要进一步研究来确定其作为治疗剂的安全性和疗效。
