Department of Biotechnology, National Formosa University, Huwei District, Yunlin, Taiwan.
Department of Research and Development, Challenge Bioproducts Co., Ltd., Yunlin, Taiwan.
BMC Complement Altern Med. 2018 Jul 3;18(1):202. doi: 10.1186/s12906-018-2267-9.
Royal jelly (RJ), the exclusive food for the larva of queen honeybee, is regarded as the novel supplement to promote human health. The function of RJ may be attributed to its major and unique fatty acid, 10-hydroxy-2-decenoic acid (10-HDA). The current study investigated the anti-inflammory function of 10-HDA on human colon cancer cells, WiDr, as well as its effect on the growth of pathogenic bacterium.
The pro-inflammatory cytokines, receptor antagonist cytokine (IL-1ra) and nuclear factor-kappa B (NF-κB) in WiDr cells was analyzed by Enzyme-linked immunosorbent assay (ELISA) or western blot. The growth inhibition of 10-HDA on bacterium was evaluated by determination of minimal inhibitory concentrations (MIC) and minimal bactericide concentrations (MBC).
The production of pro-inflammatory cytokines, Interleukin (IL)-8, IL-1β and tumor necrosis factor-alpha (TNF-α) in WiDr cells was modulated by 10-HDA. IL-8 were dramatically declined by 10-HDA at 3 mM, while IL-1β and TNF-α were significantly decreased. 10-HDA increased IL-1ra in a dose manner. NF-κB pathway is primarily in response to prototypical pro-inflammatory cytokines, and NF-κB was reduced after 10-HDA treatment. 10-HDA acted as potent bactericide against animal- or human-specific pathogens, including Staphylococcus aureus, Streptococcus alactolyticus, Staphylococcus intermedius B, Staphylococcus xylosus, Salmonella cholearasuis, Vibro parahaemolyticus and Escherichia coli (hemolytic).
The current study showed that in vitro 10-HDA from RJ exhibited anti-inflammatory activity in WiDr cells, as well as anti-bacterial activity against animal pathogens. 10-HDA showed its potential as anti-imflammtory agent and bactericide to benefit human gastrointestinal tract.
蜂王浆（RJ）是蜂王幼虫的专用食物，被认为是促进人类健康的新型补充剂。RJ 的功能可能归因于其主要且独特的脂肪酸 10-羟基-2-癸烯酸（10-HDA）。本研究探讨了 10-HDA 对人结肠癌细胞 WiDr 的抗炎作用及其对致病菌生长的影响。
通过酶联免疫吸附试验（ELISA）或 Western blot 分析 WiDr 细胞中的促炎细胞因子、受体拮抗剂细胞因子（IL-1ra）和核因子-κB（NF-κB）。通过测定最小抑菌浓度（MIC）和最小杀菌浓度（MBC）评估 10-HDA 对细菌的生长抑制作用。
10-HDA 调节 WiDr 细胞中促炎细胞因子白细胞介素（IL）-8、IL-1β和肿瘤坏死因子-α（TNF-α）的产生。10-HDA 在 3mM 时显著下调 IL-8，而 IL-1β和 TNF-α显著减少。10-HDA 以剂量依赖性方式增加 IL-1ra。NF-κB 途径主要响应典型的促炎细胞因子，10-HDA 处理后 NF-κB 减少。10-HDA 对动物或人类特异性病原体（包括金黄色葡萄球菌、无乳链球菌、中间葡萄球菌 B、木糖葡萄球菌、鼠伤寒沙门氏菌、副溶血性弧菌和大肠杆菌（溶血））具有很强的杀菌作用。
本研究表明，RJ 中的体外 10-HDA 在 WiDr 细胞中表现出抗炎活性，并且对动物病原体具有抗菌活性。10-HDA 显示出作为抗炎剂和杀菌剂的潜力，可有益于人类胃肠道。
