Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany.
Section Mass Spectrometry and Proteomics, Diagnostic Center, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany.
Int J Mol Sci. 2023 Aug 11;24(16):12684. doi: 10.3390/ijms241612684.
Tff1 is a typical gastric peptide secreted together with the mucin, Muc5ac. -deficient () mice are well known for their prominent gastric phenotype and represent a recognized model for antral tumorigenesis. Notably, intestinal abnormalities have also been reported in the past in these animals. Here, we have compared the expression of selected genes in mice and their corresponding wild-type littermates (RT-PCR analyses), focusing on different mucosal protection systems along the murine intestine. As hallmarks, genes were identified with maximum expression in the proximal colon and/or the duodenum: , //, , , , , /, . This is indicative of different protection systems such as Tff2/Muc6, Tff1-Fcgbp, gastrokines, fucosylation, and reactive oxygen species (ROS) in the proximal colon and/or duodenum. Few significant transcriptional changes were observed in the intestine of mice when compared with wild-type littermates, (), , , , . We also analyzed the expression of , , and in the pancreas, liver, and lung of and wild-type animals, indicating a cross-regulation of gene expression. Furthermore, on the protein level, heteromeric Tff1-Fcgbp and various monomeric Tff1 forms were identified in the duodenum and a high-molecular-mass Tff2/Muc6 complex was identified in the proximal colon (FPLC, proteomics).
Tff1 是一种与粘蛋白 Muc5ac 一起分泌的典型胃肽。-缺陷 () 小鼠以其突出的胃表型而闻名，是公认的胃肿瘤发生模型。值得注意的是，过去也有报道称这些动物存在肠道异常。在这里，我们比较了 小鼠及其相应的野生型同窝仔鼠（RT-PCR 分析）中选定基因的表达，重点是沿小鼠肠道的不同黏膜保护系统。作为特征，确定了在近端结肠和/或十二指肠中具有最大表达的基因： 、 // 、 、 、 、 / 、 。这表明存在不同的保护系统，例如近端结肠和/或十二指肠中的 Tff2/Muc6、Tff1-Fcgbp、胃动素、岩藻糖基化和活性氧 (ROS)。与野生型同窝仔鼠相比， 小鼠的肠道中观察到的转录变化很少， （）、 、 、 。我们还分析了 胰腺、肝脏和肺中 和野生型动物的 、 和 的表达，表明 基因表达的交叉调节。此外，在蛋白水平上，在十二指肠中鉴定出异源二聚体 Tff1-Fcgbp 和各种单体 Tff1 形式，在近端结肠中鉴定出高分子量 Tff2/Muc6 复合物（FPLC、蛋白质组学）。
