西花蓟马对菜豆中 6 种植物挥发性有机化合物的选择行为及其 OBP-转录反应

Selection Behavior and OBP-Transcription Response of Western Flower Thrips, , to Six Plant VOCs from Kidney Beans.

机构信息

Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

出版信息

Int J Mol Sci. 2023 Aug 14;24(16):12789. doi: 10.3390/ijms241612789.

DOI:10.3390/ijms241612789

PMID:37628969

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10454143/

Abstract

Plant volatile organic compounds (VOCs) are an important link that mediates chemical communication between plants and plants, plants and insects, and plants and natural enemies of insect pests. In this study, we tested the response in the selective behavior of western flower thrips, , to the VOCs of kidney bean, L., to explore their "attraction" or "repellent" effects regarding their application in integrated pest management (i.e., IPM). The results indicated that 12.7 μL/mL (E, E, E, E)-squalene, 3.2 μL/mL dioctyl phthalate, and 82.2 μL/mL ethyl benzene had a significantly attractive effect on the selective behavior of , while 10.7 μL/mL and 21.4 μL/mL 2,6-ditert-butyl-4-methyl phenol had a significantly repulsive effect on the selective behavior of , showing that responds differently to specific concentrations of VOCs from plant emissions. Interestingly, the three compounds with the specific above concentrations, after being mixed in pairs, significantly attracted compared to the control treatment; however, the mixture with the three above compounds had no significant different effect on compared to the control treatment. It can be seen that the effect with the mixtures of three kinds of VOCs had the same function and may not get better. Simultaneously, the reasons for this result from the transcription levels of odorant-binding protein genes (OBPs) were determined. There were differences in the types and transcription levels of OBPs, which played a major role in the host selection behavior of under the mixed treatment of different VOCs. It is presumed that there are specific VOCs from . plants that have a good repellent or attracting effect on the selective behavior of . , which can be used for the development of plant-derived insect attractants and repellents to serve as IPM in fields. But attention should be paid to the antagonism between plant-derived preparations and VOCs produced by plants themselves after application.

摘要

植物挥发性有机化合物（VOCs）是介导植物间、植物与昆虫间以及植物与害虫天敌间化学通讯的重要环节。本研究以西方花蓟马（Frankliniella occidentalis）为对象，测试了其对菜豆 VOCs 的选择性行为的反应，以探讨其在害虫综合治理（即 IPM）中的“引诱”或“驱避”作用。结果表明，12.7 μL/mL（E，E，E，E）-角鲨烯、3.2 μL/mL 邻苯二甲酸二辛酯和 82.2 μL/mL 乙苯对选择性行为有显著的吸引作用，而 10.7 μL/mL 和 21.4 μL/mL 2,6-二叔丁基-4-甲基苯酚对选择性行为有显著的驱避作用，表明对植物排放的特定浓度 VOCs 的反应不同。有趣的是，上述三种特定浓度的化合物混合配对后，与对照处理相比，对有显著的吸引力；然而，与对照处理相比，这三种化合物的混合物对没有显著的不同影响。可以看出，三种 VOCs 混合物的效果相同，可能不会更好。同时，从气味结合蛋白基因（OBPs）的转录水平确定了产生这种结果的原因。OBPs 的类型和转录水平存在差异，在不同 VOCs 的混合处理下，对宿主选择行为起着主要作用。推测植物可能存在特定的 VOCs，对选择性行为具有良好的驱避或吸引作用，可用于开发植物源昆虫引诱剂和驱避剂，作为田间 IPM 的手段。但应注意应用后植物源制剂与植物自身产生的 VOCs 之间的拮抗作用。