State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2023 Aug 16;24(16):12845. doi: 10.3390/ijms241612845.
Lodging is one of the most important factors affecting the high and stable yield of wheat worldwide. Solid-stemmed wheat has higher stem strength and lodging resistance than hollow-stemmed wheat does. There are many solid-stemmed varieties, landraces, and old varieties of durum wheat. However, the transfer of solid stem genes from durum wheat is suppressed by a suppressor gene located on chromosome 3D in common wheat, and only hollow-stemmed lines have been created. However, synthetic hexaploid wheat can serve as a bridge for transferring solid stem genes from tetraploid wheat to common wheat. In this study, the F, F, and F generations of a cross between solid-stemmed Syn-SAU-119 and semisolid-stemmed Syn-SAU-117 were developed. A single dominant gene, which was tentatively designated and suppresses stem solidity, was identified in synthetic hexaploid wheat Syn-SAU-117 by using genetic analysis. By using bulked segregant RNA-seq (BSR-seq) analysis, was mapped to chromosome 7DS and flanked by markers and within a 4.53 cM genetic interval corresponding to 3.86 Mb and 2.29 Mb physical regions in the Chinese Spring (IWGSC RefSeq v1.1) and (AL8/78 v4.0) genomes, respectively, in which three genes related to solid stem development were annotated. differed from a previously reported solid stem suppressor gene based on its origin and position. would provide a valuable example for research on the suppression phenomenon. The flanking markers developed in this study might be useful for screening accessions with no suppressor gene () to develop more synthetic hexaploid wheat lines for the breeding of lodging resistance in wheat and further cloning the suppressor gene .
lodging 是影响全球小麦高产稳产的最重要因素之一。实心茎小麦比空心茎小麦具有更高的茎秆强度和抗倒伏能力。硬质小麦有许多实心品种、地方品种和老品种。然而，硬质小麦的实心茎基因的转移受到普通小麦 3D 染色体上一个抑制基因的抑制，只能创建空心茎系。然而，合成六倍体小麦可以作为一个桥梁，将四倍体小麦的实心茎基因转移到普通小麦中。在这项研究中，开发了实心茎 Syn-SAU-119 和半实心茎 Syn-SAU-117 之间杂交的 F1、F2 和 F3 代。通过遗传分析，在合成六倍体小麦 Syn-SAU-117 中鉴定出一个单一显性基因，该基因暂命名为 ，并抑制茎的实心度。通过使用 bulked segregant RNA-seq (BSR-seq) 分析，将 定位到 7DS 染色体上，并由标记 和 侧翼，在 3.86Mb 和 2.29Mb 的物理区域内，分别对应于中国春（IWGSC RefSeq v1.1）和 （AL8/78 v4.0）基因组中的 4.53 cM 遗传区间，其中注释了三个与实心茎发育相关的基因。 与以前报道的实心茎抑制基因在起源和位置上不同。 为抑制现象的研究提供了一个有价值的例子。本研究中开发的侧翼标记可能有助于筛选没有抑制基因（）的 材料，以开发更多的合成六倍体小麦系，用于小麦抗倒伏性的选育，并进一步克隆抑制基因 。
