Department of Internal Medicine III, Giessen University Hospital, Klinikstrasse 33, 35392 Giessen, Germany.
Int J Mol Sci. 2023 Aug 17;24(16):12901. doi: 10.3390/ijms241612901.
Recent investigation has revealed the significant role of Cathelicidin antimicrobial peptide (CAMP) in infection defense and innate immunity processes in adipose tissue. Meanwhile, knowledge of its regulation and functions in metabolic contexts as an adipokine remains sparce. The present study investigated the postprandial regulation of circulating CAMP levels during oral glucose tolerance tests (OGTTs). Eighty-six metabolically healthy volunteers participated in a standardized 75 g-2 h-OGTT setting. The effects of exogenous glucose, insulin, and incretins on expression in human adipocyte culture (cell-line SGBS) were studied in vitro. CAMP concentrations in blood serum samples were measured by ELISA techniques and adipocyte gene expression levels were quantified by real-time PCR. Of note, base-line CAMP serum quantities were negatively correlated with HDL cholesterol levels as well as with the anti-inflammatory adipokine adiponectin. During the 2 h following glucose ingestion, a significant rise in circulating CAMP concentrations was observed in considerable contrast to reduced quantities of fatty acid binding proteins (FABP) 2 and 4 and dipeptidyl peptidase 4 (DPP4). In SGBS adipocytes, neither differing glucose levels nor insulin or incretin treatment significantly induced mRNA levels. According to our data, glucose represents a positive postprandial regulator of systemic CAMP. This effect apparently is not mediated by the regulatory impact of glucose metabolism on adipocyte expression.
最近的研究揭示了 Cathelicidin 抗菌肽 (CAMP) 在脂肪组织感染防御和先天免疫过程中的重要作用。同时，作为一种脂肪细胞因子，其在代谢环境中的调节和功能的相关知识仍然很少。本研究调查了口服葡萄糖耐量试验 (OGTT) 期间循环 CAMP 水平的餐后调节。86 名代谢健康的志愿者参加了标准化的 75 g-2 h-OGTT 试验。在体外研究了外源性葡萄糖、胰岛素和肠促胰岛素对人脂肪细胞培养 (细胞系 SGBS) 中 表达的影响。通过 ELISA 技术测量血清样本中的 CAMP 浓度，并通过实时 PCR 定量测定脂肪细胞基因表达水平。值得注意的是，基线 CAMP 血清量与高密度脂蛋白胆固醇水平以及抗炎性脂肪细胞因子脂联素呈负相关。与脂肪酸结合蛋白 (FABP) 2 和 4 以及二肽基肽酶 4 (DPP4) 的减少量相比，在摄入葡萄糖后 2 小时内，循环 CAMP 浓度显著升高。在 SGBS 脂肪细胞中，葡萄糖水平的差异、胰岛素或肠促胰岛素处理均未显著诱导 mRNA 水平。根据我们的数据，葡萄糖是全身 CAMP 的正餐后调节因子。这种作用显然不是通过葡萄糖代谢对脂肪细胞 表达的调节作用介导的。
