Patuhai Aicah, Wahab Puteri Edaroyati Megat, Yusoff Martini Mohammad, Dewir Yaser Hassan, Alsughayyir Ali, Hakiman Mansor
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Plants (Basel). 2023 Aug 15;12(16):2953. doi: 10.3390/plants12162953.
(Burm.f.) Wall. (Acanthaceae) is revered for its medicinal properties. In vitro culture of medicinal plants has assisted in improving both the quantity and quality of their yield. The current study investigated the effects of different surface sterilization treatments, plant growth regulators (PGRs), and elicitors on culture establishment and axillary shoot multiplication of . Subsequently, the production of andrographolide in the in vitro plantlets was evaluated using high-performance liquid chromatography (HPLC) analysis. The shoot-tip explant was successfully sterilized using 60% commercial bleach for 5 min of immersion with a 90% survival rate and 96.67% aseptic culture. The optimal PGR for shoot growth was 6-benzylaminopurine (BAP) at 17.76 µM, supplemented into Murashige and Skoog (MS) media, producing 23.57 ± 0.48 leaves, 7.33 ± 0.10 shoots, and a 3.06 ± 0.02 cm length of shoots. Subsequently, MS medium supplemented with 5 mg/L chitosan produced 26.07 ± 0.14 leaves, 8.33 ± 0.07 shoots, and a 3.63 ± 0.02 cm length of shoots. The highest andrographolide content was obtained using the plantlets harvested from 5 mg/L chitosan with 2463.03 ± 0.398 µg/mL compared to the control (without elicitation) with 256.73 ± 0.341 µg/mL (859.39% increase). The results imply that the protocol for the shoot-tip culture of was developed, and that elicitation enhanced the herbage yield and the production of andrographolide.
（缅甸）瓦尔（爵床科）因其药用特性而备受推崇。药用植物的离体培养有助于提高其产量的数量和质量。本研究调查了不同表面灭菌处理、植物生长调节剂（PGRs）和诱导子对[植物名称]培养建立和腋芽增殖的影响。随后，使用高效液相色谱（HPLC）分析评估离体苗中穿心莲内酯的产量。茎尖外植体用60%商业漂白剂浸泡5分钟成功灭菌，存活率为90%，无菌培养率为96.67%。茎生长的最佳植物生长调节剂是17.76µM的6-苄基腺嘌呤（BAP），添加到Murashige和Skoog（MS）培养基中，产生23.57±0.48片叶子、7.33±0.10个芽和3.06±0.02厘米长的芽。随后，添加5mg/L壳聚糖的MS培养基产生26.07±0.14片叶子、8.33±0.07个芽和3.63±0.02厘米长的芽。使用从5mg/L壳聚糖收获的苗获得的穿心莲内酯含量最高，为2463.03±0.398µg/mL，而对照（无诱导）为256.73±0.341µg/mL（增加859.39%）。结果表明，已开发出[植物名称]茎尖培养方案，诱导提高了牧草产量和穿心莲内酯的产量。
