Tian Shifu, Liu Bojing, Shen Yanan, Cao Shasha, Lai Yinyan, Lu Guodong, Wang Zonghua, Wang Airong
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2023 Aug 16;12(16):2965. doi: 10.3390/plants12162965.
is a devastating fungal pathogen that causes severe economic losses in global tomato cultivation. Understanding the molecular mechanisms driving tomatoes' response to this pathogen is crucial for developing effective strategies to counter it. Although the Micro-Tom (MT) cultivar has been used as a model, its stage-specific response to remains poorly understood. In this study, we examined the response of the MT and Ailsa Craig (AC) cultivars to at different time points (12-48 h post-infection (hpi)). Our results indicated that MT exhibited a stronger resistant phenotype at 18-24 hpi but became more susceptible to later (26-48 hpi) compared to AC. Transcriptome analysis revealed differential gene expression between MT at 24 hpi and AC at 22 hpi, with MT showing a greater number of differentially expressed genes (DEGs). Pathway and functional annotation analysis revealed significant differential gene expression in processes related to metabolism, biological regulation, detoxification, photosynthesis, and carbon metabolism, as well as some immune system-related genes. MT demonstrated an increased reliance on Ca pathway-related proteins, such as CNGCs, CDPKs, and CaMCMLs, to resist invasion. infection induced the activation of PTI, ETI, and SA signaling pathways, involving the modulation of various genes such as FLS2, BAK1, CERK1, RPM, SGT1, and EDS1. Furthermore, transcription factors such as WRKY, MYB, NAC, and AUX/IAA families played crucial regulatory roles in tomatoes' defense against . These findings provide valuable insights into the molecular mechanisms underlying tomatoes' defense against and offer potential strategies to enhance plant resistance.
是一种具有毁灭性的真菌病原体，在全球番茄种植中造成严重经济损失。了解驱动番茄对这种病原体作出反应的分子机制，对于制定有效的应对策略至关重要。尽管微型番茄（MT）品种已被用作模型，但其对[病原体名称未给出]的阶段特异性反应仍知之甚少。在本研究中，我们在不同时间点（感染后12 - 48小时（hpi））检测了MT和艾尔莎·克雷格（AC）品种对[病原体名称未给出]的反应。我们的结果表明，与AC相比，MT在18 - 24 hpi表现出更强的抗性表型，但在后期（26 - 48 hpi）对[病原体名称未给出]更敏感。转录组分析揭示了MT在24 hpi和AC在22 hpi之间的差异基因表达，MT显示出更多的差异表达基因（DEG）。通路和功能注释分析揭示了在与代谢、生物调节、解毒、光合作用和碳代谢相关的过程以及一些免疫系统相关基因中存在显著的差异基因表达。MT表现出对钙途径相关蛋白（如CNGC、CDPK和CaMCML）的依赖性增加，以抵抗[病原体名称未给出]的入侵。[病原体名称未给出]感染诱导了PTI、ETI和SA信号通路的激活，涉及对各种基因（如FLS2、BAK1、CERK1、RPM、SGT1和EDS1）的调控。此外，转录因子如WRKY、MYB、NAC和AUX/IAA家族在番茄对[病原体名称未给出]的防御中发挥了关键的调控作用。这些发现为番茄抵御[病原体名称未给出]的分子机制提供了有价值的见解，并提供了增强植物抗性的潜在策略。
