Vermeulen N M, Gothe R, Senekal A C, Neitz A W
Onderstepoort J Vet Res. 1986 Sep;53(3):147-52.
Major differences were observed in hexane, ethanol and butanol extracts of eggs obtained from Rhipicephalus evertsi evertsi females in which the porose areas functioned normally (AP+ eggs) and from R. evertsi evertsi in which the porose areas were selectively destroyed by electrocautery (AP- eggs). Mass yields and UV spectra of the hexane extracts were similar for AP+ and AP- eggs. The UV spectra changed only slightly in the 294-320 nm range with respect to time and temperature. High performance liquid chromatography revealed 2 components which originate from the porose areas. Mass spectroscopy of these components indicated the presence of aliphatic and phenolic groups. The ethanol and butanol extracts showed quantitative but no qualitative differences with respect to AP+ and AP- eggs. Electrophoretic fractionation of the butanol extracts revealed the presence of proteins in the secretion of the porose areas. Apart from this information on the chemical composition of the secretion, no indication was obtained of their function during oviposition of R. evertsi evertsi.
在采采蝇（Rhipicephalus evertsi evertsi）正常具有多孔区功能的雌性采采蝇（AP +卵）和多孔区被电烙术选择性破坏的采采蝇（AP-卵）所产的卵的己烷、乙醇和丁醇提取物中观察到了主要差异。AP +和AP-卵的己烷提取物的质量产量和紫外光谱相似。紫外光谱在294-320nm范围内仅随时间和温度略有变化。高效液相色谱显示有两种成分来自多孔区。这些成分的质谱分析表明存在脂肪族和酚类基团。乙醇和丁醇提取物在AP +和AP-卵方面显示出定量差异，但没有定性差异。丁醇提取物的电泳分级显示多孔区分泌物中存在蛋白质。除了有关分泌物化学成分的这些信息外，未获得它们在采采蝇产卵过程中功能的迹象。
