Department of Dermatology, The First Affiliated Hospital of Guangxi Medical University, Nanning, The Guangxi Zhuang Autonomous Region, 530021, China.
Department of Dermatology, The Second Nanning People's Hospital, Nanning, The Guangxi Zhuang Autonomous Region, 530031, China.
Anal Biochem. 2023 Nov 1;680:115299. doi: 10.1016/j.ab.2023.115299. Epub 2023 Aug 25.
MicroRNAs (miRNAs) play a significant role in regulating diverse physiological processes, and are regarded as novel diagnostic biomarkers. However, the sensitive and reliable miRNA detection remains a huge challenge. Herein, we propose a proximity ligated initiated magnesium ion (Mg)-dependent DNAzyme-powered signal cascade for sensitive, accurate and reliable detection of miRNAs. Three signal amplification processes are involved in this approach, including the target miRNA recycle, DNAzyme powered substrate cleavage, and catalytic hairpin reaction (CHA). Based on this, the approach shows a low limit of detection of 523 aM and a wide detection range of 7 orders of magnitudes, which is comparable or superior to most of the former miRNA detection methods. In addition, the approach also possesses a high selectivity to target miRNA, suggesting a potential promising future of the approach for rapid detection of miRNAs in the application of developing novel tools for skin cancer diagnosis, and recovery evaluation.
微小 RNA（miRNAs）在调节多种生理过程中发挥着重要作用，被认为是新型诊断生物标志物。然而，灵敏且可靠的 miRNA 检测仍然是一个巨大的挑战。在此，我们提出了一种基于邻近连接引发的镁离子（Mg）依赖性 DNA 酶动力信号级联反应，用于灵敏、准确和可靠地检测 miRNA。该方法涉及三个信号放大过程，包括目标 miRNA 的循环、DNA 酶动力底物切割和催化发夹反应（CHA）。基于此，该方法的检测下限低至 523 aM，检测范围宽达 7 个数量级，与大多数以前的 miRNA 检测方法相当或更优。此外，该方法还对目标 miRNA 具有高选择性，这表明该方法在开发用于皮肤癌诊断和恢复评估的新型工具的 miRNA 快速检测方面具有广阔的应用前景。
