State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Sci Total Environ. 2023 Dec 1;902:166569. doi: 10.1016/j.scitotenv.2023.166569. Epub 2023 Aug 24.
Structurally diverse per- and polyfluoroalkyl substances (PFASs) are increasingly detected in ecosystems and humans. Therefore, the clarification of their ecological and health risks is urgently required. In the present study, the toxicity of a series of PFASs, including PFOS, PFBS, Nafion BP1, Nafion BP2, F53B, OBS, PFOA, PFUnDA, PFO5DoDA, HFPO-TA was investigated. Similarities and differences in the developmental toxicity potentials were revealed. Our results demonstrated that PFUnDA exhibited the highest toxicity with the lowest EC value of 4.36 mg/L (for morphological abnormality); this was followed by F53B (5.58 mg/L), PFOS (6.15 mg/L), and OBS (10.65 mg/L). Positive correlations with volatility/solubility and chemotypes related to specific biological activity, including the bioconcentration factor (LogBCF), and negative correlations with lipid solubility and carbon chain component-related chemotypes, including the number of carbon and fluorine atoms, provided a reasonable explanation in the view of molecular structures. Furthermore, comparative transcriptome analysis provided molecular evidence for the relationship between PFASs exposure and malformations. Common differentially expressed genes (DEGs) involved in spine curve development, pericardial edema, and cell/organism growth-related pathways presented common targets, leading to toxic effects. Therefore, the present results provide novel insights into the potential environmental risks of structurally diverse PFASs and contribute to the selection of safer PFAS replacements.
结构多样的全氟和多氟烷基物质（PFASs）在生态系统和人类中越来越多地被检测到。因此，迫切需要阐明其生态和健康风险。本研究考察了一系列 PFASs 的毒性，包括 PFOS、PFBS、Nafion BP1、Nafion BP2、F53B、OBS、PFOA、PFUnDA、PFO5DoDA、HFPO-TA。揭示了它们在发育毒性潜力方面的异同。研究结果表明，PFUnDA 表现出最高的毒性，其形态异常的最低 EC 值为 4.36mg/L；其次是 F53B（5.58mg/L）、PFOS（6.15mg/L）和 OBS（10.65mg/L）。与挥发性/溶解度和与特定生物活性相关的化学型（包括生物浓缩因子 LogBCF）呈正相关，与脂溶性和与碳链成分相关的化学型（包括碳原子和氟原子数量）呈负相关，从分子结构的角度提供了合理的解释。此外，比较转录组分析为 PFASs 暴露与畸形之间的关系提供了分子证据。涉及脊柱曲线发育、心包水肿和细胞/生物体生长相关途径的常见差异表达基因（DEGs）呈现出共同的靶点，导致毒性作用。因此，本研究结果为结构多样的 PFASs 的潜在环境风险提供了新的见解，并有助于选择更安全的 PFAS 替代品。
