Afsharipour Sepehr, Kavianipoor Samane, Ranjbar Mehdi, Bagheri Amir Mohammad, Lari Najafi Moslem, Banat Ibrahim M, Ohadi Mandana, Dehghannoudeh Gholamreza
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Ann Pharm Fr. 2023 Nov;81(6):968-976. doi: 10.1016/j.pharma.2023.08.008. Epub 2023 Aug 24.
Nanofibers are a class of nanomaterial with specific physicochemical properties and characteristics making them quite sought after and investigated by researchers. Lipopeptide biosurfactant (LPB) formulation properties were previously established in wound healing. LPB were isolated from in vitro culture of Acinetobacter junii B6 and loaded on nanofibers formulation produced by electrospinning method with different ratios of carboxymethylcellulose (CMC), polyvinyl alcohol (PVA), and Poloxamer. Numerous experimental control tests were carried out on formulations, including physicochemical properties which were evaluated by using dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), morphology study by scanning electron microscopy (SEM), and thermal stability. The best nanofibers formulation was obtained by the electrospinning method, with a voltage of 19.8 volts, a discharge capacity of 1cm/h, a cylindrical rotating velocity of 100rpm, and a needle interval of 7cm from the cylinder, which continued for 7hours. The formulation contained 2% (w/v) CMC, 10% (w/v) poloxamer, 9% (w/v) PVA, and 5% (w/v) LPB. This formula had desirable physicochemical properties including spreadability, stability, and uniformity with the particle size of about 590nm.
纳米纤维是一类具有特定物理化学性质和特征的纳米材料，这使得它们备受研究人员的追捧和研究。脂肽生物表面活性剂（LPB）的配方特性先前已在伤口愈合方面得到确立。LPB是从琼氏不动杆菌B6的体外培养物中分离出来的，并负载于通过静电纺丝法制备的纳米纤维配方中，该配方含有不同比例的羧甲基纤维素（CMC）、聚乙烯醇（PVA）和泊洛沙姆。对这些配方进行了大量实验对照测试，包括使用动态光散射（DLS）评估的物理化学性质、傅里叶变换红外光谱（FT-IR）、通过扫描电子显微镜（SEM）进行的形态学研究以及热稳定性测试。通过静电纺丝法获得了最佳的纳米纤维配方，其电压为19.8伏，放电容量为1厘米/小时，圆柱旋转速度为100转/分钟，针与圆柱的间距为7厘米，持续时间为7小时。该配方包含2%（w/v）的CMC、10%（w/v）的泊洛沙姆、9%（w/v）的PVA和5%（w/v）的LPB。该配方具有理想的物理化学性质，包括铺展性、稳定性和均匀性，粒径约为590纳米。
