在人类成纤维细胞中，DNA复制及紫外线诱导的DNA修复合成对丁基苯基脱氧鸟苷三磷酸抑制作用的敏感性远低于DNA聚合酶α。
DNA replication and UV-induced DNA repair synthesis in human fibroblasts are much less sensitive than DNA polymerase alpha to inhibition by butylphenyl-deoxyguanosine triphosphate.
作者信息
Dresler S L, Frattini M G
出版信息
Nucleic Acids Res. 1986 Sep 11;14(17):7093-102. doi: 10.1093/nar/14.17.7093.
Abstract
In mammalian cells, both semiconservative DNA replication and the DNA repair patch synthesis induced by high doses of ultraviolet radiation are known to be inhibited by aphidicolin, indicating the involvement in these processes of one or both of the aphidicolin-sensitive DNA polymerases, alpha and/or delta. In this paper, N2-(p-n-butylphenyl)-2'-deoxyguanosine-5'-triphosphate, a strong inhibitor of polymerase alpha and a weak inhibitor of polymerase delta, is used to further characterize the DNA polymerase(s) involved in these two forms of nuclear DNA synthesis. In permeable human fibroblasts, DNA replication and ultraviolet-induced DNA repair synthesis are more resistant to the inhibitor than DNA polymerase alpha by factors of approximately 500 and 3000, respectively. These findings are most consistent with the involvement of DNA polymerase delta in these processes.
摘要
在哺乳动物细胞中，已知高剂量紫外线辐射诱导的半保留DNA复制和DNA修复补丁合成均受阿非迪霉素抑制，这表明阿非迪霉素敏感的DNA聚合酶α和/或δ中的一种或两种参与了这些过程。在本文中，N2-（对正丁基苯基）-2'-脱氧鸟苷-5'-三磷酸作为聚合酶α的强抑制剂和聚合酶δ的弱抑制剂，被用于进一步表征参与这两种核DNA合成形式的DNA聚合酶。在可渗透的人成纤维细胞中，DNA复制和紫外线诱导的DNA修复合成对该抑制剂的抗性分别比DNA聚合酶α高约500倍和3000倍。这些发现与DNA聚合酶δ参与这些过程最为一致。
相似文献
1
DNA replication and UV-induced DNA repair synthesis in human fibroblasts are much less sensitive than DNA polymerase alpha to inhibition by butylphenyl-deoxyguanosine triphosphate.在人类成纤维细胞中，DNA复制及紫外线诱导的DNA修复合成对丁基苯基脱氧鸟苷三磷酸抑制作用的敏感性远低于DNA聚合酶α。
Nucleic Acids Res. 1986 Sep 11;14(17):7093-102. doi: 10.1093/nar/14.17.7093.
2
Analysis of butylphenyl-guanine, butylphenyl-deoxyguanosine, and butylphenyl-deoxyguanosine triphosphate inhibition of DNA replication and ultraviolet-induced DNA repair synthesis using permeable human fibroblasts.使用可渗透的人成纤维细胞分析丁基苯基鸟嘌呤、丁基苯基脱氧鸟苷和丁基苯基脱氧鸟苷三磷酸对DNA复制和紫外线诱导的DNA修复合成的抑制作用。
Biochem Pharmacol. 1988 Mar 15;37(6):1033-7. doi: 10.1016/0006-2952(88)90506-0.
3
DNA polymerase delta mediates excision repair in growing cells damaged with ultraviolet radiation.
Biochem Cell Biol. 1991 Apr;69(4):303-8. doi: 10.1139/o91-046.
4
Effect of DNA polymerase inhibitors on DNA repair in intact and permeable human fibroblasts: evidence that DNA polymerases delta and beta are involved in DNA repair synthesis induced by N-methyl-N'-nitro-N-nitrosoguanidine.DNA聚合酶抑制剂对完整及通透的人成纤维细胞DNA修复的影响：DNA聚合酶δ和β参与N-甲基-N'-硝基-N-亚硝基胍诱导的DNA修复合成的证据
Biochemistry. 1990 Jan 9;29(1):286-91. doi: 10.1021/bi00453a039.
5
Involvement of DNA polymerase delta in DNA repair synthesis in human fibroblasts at late times after ultraviolet irradiation.紫外线照射后晚期人成纤维细胞中DNA聚合酶δ参与DNA修复合成。
Biochemistry. 1988 Aug 23;27(17):6379-83. doi: 10.1021/bi00417a028.
6
Bleomycin-induced DNA repair synthesis in permeable human fibroblasts: mediation of long-patch and short-patch repair by distinct DNA polymerases.博来霉素诱导的可渗透人成纤维细胞中的DNA修复合成：不同DNA聚合酶对长片段和短片段修复的介导作用
Biochemistry. 1989 Nov 28;28(24):9515-20. doi: 10.1021/bi00450a040.
7
Does butylphenyl-deoxyguanosine triphosphate differentially inhibit DNA polymerase alpha and delta activities in permeabilized HeLa cells?丁基苯基脱氧鸟苷三磷酸是否能在通透的HeLa细胞中差异抑制DNA聚合酶α和δ的活性？
Nucleic Acids Res. 1990 Feb 25;18(4):753-8. doi: 10.1093/nar/18.4.753.
8
2',3'-Dideoxythymidine 5'-triphosphate inhibition of DNA replication and ultraviolet-induced DNA repair synthesis in human cells: evidence for involvement of DNA polymerase delta.2',3'-双脱氧胸苷5'-三磷酸对人细胞DNA复制及紫外线诱导的DNA修复合成的抑制作用：DNA聚合酶δ参与的证据
Biochemistry. 1987 May 19;26(10):2664-8. doi: 10.1021/bi00384a002.
9
Involvement of DNA polymerase delta and/or epsilon in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin).
Biochim Biophys Acta. 1994 Oct 18;1219(2):302-6. doi: 10.1016/0167-4781(94)90052-3.
10
In situ enzymology of DNA replication and ultraviolet-induced DNA repair synthesis in permeable human cells.可渗透人细胞中DNA复制及紫外线诱导的DNA修复合成的原位酶学
Biochemistry. 1988 Sep 20;27(19):7247-54. doi: 10.1021/bi00419a011.
引用本文的文献
1
Cellular proteins required for adeno-associated virus DNA replication in the absence of adenovirus coinfection.在无腺病毒共感染情况下腺相关病毒DNA复制所需的细胞蛋白。
J Virol. 1998 Apr;72(4):2777-87. doi: 10.1128/JVI.72.4.2777-2787.1998.
2
Involvement of the yeast DNA polymerase delta in DNA repair in vivo.酵母DNA聚合酶δ参与体内DNA修复。
Genetics. 1997 Aug;146(4):1239-51. doi: 10.1093/genetics/146.4.1239.
3
The 3'-5' exonuclease of human DNA polymerase delta (pol delta) is regulated by pol delta accessory factors and deoxyribonucleoside triphosphates.人类DNA聚合酶δ（pol δ）的3'-5'核酸外切酶受pol δ辅助因子和脱氧核糖核苷三磷酸的调控。
Nucleic Acids Res. 1993 Apr 25;21(8):1935-9. doi: 10.1093/nar/21.8.1935.
4
Aphidicolin-resistant human cell lines resistant to UV and bleomycin.
In Vitro Cell Dev Biol. 1993 Mar;29A(3 Pt 1):195-8. doi: 10.1007/BF02634182.
5
Strategic down-regulation of DNA polymerase beta by antisense RNA sensitizes mammalian cells to specific DNA damaging agents.通过反义RNA对DNA聚合酶β进行策略性下调可使哺乳动物细胞对特定的DNA损伤剂敏感。
Nucleic Acids Res. 1995 Oct 11;23(19):3810-5. doi: 10.1093/nar/23.19.3810.
6
Exonucleolytic proofreading by calf thymus DNA polymerase delta.小牛胸腺DNA聚合酶δ的核酸外切酶校对作用。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4865-9. doi: 10.1073/pnas.84.14.4865.
7
Purification and characterization of porcine liver DNA polymerase gamma: utilization of dUTP and dTTP during in vitro DNA synthesis.猪肝DNA聚合酶γ的纯化与特性：体外DNA合成过程中dUTP和dTTP的利用
Nucleic Acids Res. 1988 Jun 24;16(12):5645-59. doi: 10.1093/nar/16.12.5645.
8
Arabinofuranosyl nucleosides induce common fragile sites.阿拉伯呋喃糖基核苷诱导常见脆性位点。
Hum Genet. 1988 Jun;79(2):157-62. doi: 10.1007/BF00280556.
9
The effect of drugs on mitochondrial DNA synthesis can be tested in isolated synaptosomes.药物对线粒体DNA合成的影响可以在分离的突触体中进行测试。
Nucleic Acids Res. 1986 Dec 9;14(23):9523-4. doi: 10.1093/nar/14.23.9523.
10
Changes in cyclin/proliferating cell nuclear antigen distribution during DNA repair synthesis.DNA修复合成过程中细胞周期蛋白/增殖细胞核抗原分布的变化。
J Cell Biol. 1988 Nov;107(5):1623-8. doi: 10.1083/jcb.107.5.1623.
本文引用的文献
1
Rate and extent of DNA repair in nondividing human diploid fibroblasts.非分裂人二倍体成纤维细胞中DNA修复的速率和程度。
Cancer Res. 1981 Mar;41(3):819-25.
2
Characterization of deoxyribonucleic acid repair synthesis in permeable human fibroblasts.可渗透人成纤维细胞中脱氧核糖核酸修复合成的特性分析
Biochemistry. 1982 May 11;21(10):2557-64. doi: 10.1021/bi00539a040.
3
New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha.通过一种不同寻常的DNA聚合酶α抑制剂——阿非科林揭示的真核生物DNA复制生物化学新观点。
Cell. 1981 Mar;23(3):647-8. doi: 10.1016/0092-8674(81)90426-8.
4
Butylphenyl dGTP: a selective and potent inhibitor of mammalian DNA polymerase alpha.丁基苯基dGTP：一种对哺乳动物DNA聚合酶α具有选择性和强效的抑制剂。
Nucleic Acids Res. 1984 Apr 25;12(8):3695-706. doi: 10.1093/nar/12.8.3695.
5
Synthesis and characterization of N2-(p-n-butylphenyl)-2'-deoxyguanosine and its 5'-triphosphate and their inhibition of HeLa DNA polymerase alpha.N2-(对正丁基苯基)-2'-脱氧鸟苷及其5'-三磷酸酯的合成、表征及其对HeLa DNA聚合酶α的抑制作用
J Med Chem. 1984 Feb;27(2):175-81. doi: 10.1021/jm00368a012.
6
Comparative enzymology of ultraviolet-induced DNA repair synthesis and semiconservative DNA replication in permeable diploid human fibroblasts.
J Biol Chem. 1984 Nov 25;259(22):13947-52.
7
Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.通过一种新方法对纯化至同质的小牛胸腺DNA聚合酶δ进行的进一步研究。
Biochemistry. 1984 Apr 24;23(9):1906-13. doi: 10.1021/bi00304a003.
8
Identification of DNA polymerases involved in DNA excision repair in diploid human fibroblasts.二倍体人成纤维细胞中参与DNA切除修复的DNA聚合酶的鉴定。
J Biol Chem. 1983 Aug 25;258(16):9990-4.
9
Identification of a higher molecular weight DNA polymerase alpha catalytic polypeptide in monkey cells by monoclonal antibody.利用单克隆抗体鉴定猴细胞中一种分子量更高的DNA聚合酶α催化多肽。
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7777-81. doi: 10.1073/pnas.81.24.7777.
10
DNA polymerase delta: one polypeptide, two activities.DNA聚合酶δ：一种多肽，两种活性。
Biochemistry. 1982 May 11;21(10):2513-8. doi: 10.1021/bi00539a034.
Zotero 插件