State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Int J Biol Macromol. 2023 Dec 1;252:126450. doi: 10.1016/j.ijbiomac.2023.126450. Epub 2023 Aug 25.
The estrogen receptor-positive (ER+) breast cancers constitute more than 50 % of breast cancers, seriously threatening the health of women. Unfortunately, the detection and targeted therapy of ER+ breast cancers remain a challenge. Here, a novel nucleic acid aptamer S1-4 was developed to specifically target ER+ breast cancer MCF-7 cells by using Cell-SELEX and nucleic acid truncation strategies. The affinity dissociation constant of the binding of aptamer S1-4 to MCF-7 cells was 97.6 ± 7.5 nM in vitro. Compared with HER2+ breast cells SK-BR-3 and triple-negative breast cancer cells MDA-MB-231, MCF-7 cells were selectively recognized and targeted by aptamer S1-4. Fluorescence tracing in vivo results also indicated that aptamer S1-4 selectively targeted the cell membrane of tumor tissues in MCF-7- but not in SK-BR3 or MDB-MA-231-bearing mice. This selectively developed novel aptamer probe S1-4 with high affinity could be used for the diagnosis and treatment of ER+ breast cancers.
雌激素受体阳性（ER+）乳腺癌占乳腺癌的 50%以上，严重威胁着女性的健康。不幸的是，ER+乳腺癌的检测和靶向治疗仍然是一个挑战。在这里，我们使用细胞 SELEX 和核酸截断策略开发了一种新型核酸适体 S1-4，以特异性靶向 ER+乳腺癌 MCF-7 细胞。适体 S1-4 与 MCF-7 细胞结合的亲和力解离常数为 97.6±7.5 nM。与 HER2+乳腺癌细胞 SK-BR-3 和三阴性乳腺癌细胞 MDA-MB-231 相比，适体 S1-4 选择性地识别和靶向 MCF-7 细胞。体内荧光示踪结果也表明，适体 S1-4 选择性地靶向 MCF-7 荷瘤小鼠而不是 SK-BR3 或 MDA-MA-231 荷瘤小鼠的肿瘤组织细胞膜。这种具有高亲和力的新型选择性开发的适体探针 S1-4 可用于 ER+乳腺癌的诊断和治疗。
