Ngai Hiu-Lam, Lee Hung Kay, Shaw Pang-Chui
School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, China.
Heliyon. 2023 Aug 6;9(8):e18946. doi: 10.1016/j.heliyon.2023.e18946. eCollection 2023 Aug.
DNA barcoding of herbs allows accurate species authentication. However, the DNA of herbs are often not easily PCR amplified due to co-extraction of inhibitors. Methods have been developed to improve DNA extraction to reduce contaminants. These methods usually require toxic chemical treatments or expensive commercial kits and are labor intensive. In this report, we collected the air passed from the herbs and directly amplified the DNA obtained. Results showed that DNA could be obtained, and it was PCR amplifiable. Sequencing of the amplified DNA allowed species authentication. This DNA collection method is applicable to herbs from different plant tissues. It has the advantages of reducing the use of toxic substances and more economical.
草药的DNA条形码技术可实现准确的物种鉴定。然而，由于共提取了抑制剂，草药的DNA往往不易通过PCR扩增。已开发出一些方法来改进DNA提取以减少污染物。这些方法通常需要有毒化学处理或昂贵的商业试剂盒，且劳动强度大。在本报告中，我们收集了从草药中通过的空气，并直接扩增所获得的DNA。结果表明，可以获得DNA，并且它可进行PCR扩增。对扩增后的DNA进行测序可实现物种鉴定。这种DNA收集方法适用于来自不同植物组织的草药。它具有减少有毒物质使用和更经济的优点。
