Zhang Tianjiao, Wei Shuli, Liu Yajie, Cheng Chao, Ma Jie, Yue Linfang, Gao Yanrong, Cheng Yuchen, Ren Yongfeng, Su Shaofeng, Zhao Xiaoqing, Lu Zhanyuan
School of Life Science, Inner Mongolia University, Hohhot, China.
Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China.
Front Microbiol. 2023 Aug 11;14:1167293. doi: 10.3389/fmicb.2023.1167293. eCollection 2023.
Crop straw contains huge amounts of exploitable energy, and efficient biomass degradation measures have attracted worldwide attention. Mining strains with high yields of cellulose-degrading enzymes is of great significance for developing clean energy and industrial production of related enzymes. In this study, we reported a high-quality genome sequence of SSF6 strain using high-throughput sequencing technology (Illumina PE150 and PacBio) and assessed its lignocellulose degradation potential. The results demonstrated that the genome of SSF6 was 3.89 Mb and contained 4,015 genes, of which 2,972, 3,831 and 158 genes were annotated in the COGs (Clusters of Orthologous Groups), KEGG (Kyoto Encyclopedia of Genes and Genomes) and CAZyme (Carbohydrate-Active enZymes) databases, respectively, and contained a large number of genes related to carbohydrate metabolism. Furthermore, SSF6 has a high cellulose degradation capacity, with a filter paper assay (FPA) and an exoglucanase activity of 64.48 ± 0.28 and 78.59 ± 0.42 U/mL, respectively. Comparative genomic analysis depicted that SSF6 was richer in carbohydrate hydrolase gene. In conclusion, the cellulose-degrading ability of SSF6 was revealed by genome sequencing and the determination of cellulase activity, which laid a foundation for further cellulose degradation and bioconversion.
农作物秸秆含有大量可利用的能量，高效的生物质降解措施已引起全球关注。挖掘高产纤维素降解酶的菌株对于开发清洁能源和相关酶的工业化生产具有重要意义。在本研究中，我们利用高通量测序技术（Illumina PE150和PacBio）报道了SSF6菌株的高质量基因组序列，并评估了其木质纤维素降解潜力。结果表明，SSF6的基因组为3.89 Mb，包含4015个基因，其中分别有2972、3831和158个基因在COG（直系同源簇）、KEGG（京都基因与基因组百科全书）和CAZyme（碳水化合物活性酶）数据库中得到注释，并且包含大量与碳水化合物代谢相关的基因。此外，SSF6具有较高的纤维素降解能力，滤纸酶活性（FPA）和外切葡聚糖酶活性分别为64.48±0.28和78.59±0.42 U/mL。比较基因组分析表明，SSF6的碳水化合物水解酶基因更为丰富。总之，通过基因组测序和纤维素酶活性测定揭示了SSF6的纤维素降解能力，为进一步的纤维素降解和生物转化奠定了基础。
