Department of Neurosurgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Jiangnan University, Wuxi, China.
PeerJ. 2023 Aug 21;11:e15846. doi: 10.7717/peerj.15846. eCollection 2023.
Multiple sclerosis (MS) is a chronic inflammatory neurologic disease characterized by the demyelinating injury of the central nervous system (CNS). It was reported that the mutant peptide came from myelin proteolipid protein (PLP) and myelin basic protein (MBP) might play a critical role in immunotherapy function of MS. However, endogenous peptides in demyelinating brain tissue of MS and their role in the pathologic process of MS have not been revealed. Here, we performed peptidomic analysis of freshly isolated callosum (CC) from the brains of CPZ-treated mice and normal diet controls of male C57BL/6 mice by LC-MS/MS. Identified a total of 217 peptides were expressed at different levels in MS mice model compared with controls. By performed GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis, we found that the precursor protein of these differently expressed peptides (DEPs) were associated with myelin sheath and oxidative phosphorylation. Our study is the first brain peptidomic of MS mice model, revealing the distinct features of DEPs in demyelination brain tissue. These DPEs may provide further insight into the pathogenesis and complexity of MS, which would facilitate the discovery of the potential novel and effective strategy for the treatment of MS.
多发性硬化症（MS）是一种慢性炎症性神经系统疾病，其特征是中枢神经系统（CNS）脱髓鞘损伤。据报道，来自髓鞘少突胶质细胞糖蛋白（PLP）和髓鞘碱性蛋白（MBP）的突变肽可能在 MS 的免疫治疗功能中发挥关键作用。然而，MS 脱髓鞘脑组织中的内源性肽及其在 MS 病理过程中的作用尚未被揭示。在这里，我们通过 LC-MS/MS 对 CPZ 处理的雄性 C57BL/6 小鼠和正常饮食对照的新鲜分离的胼胝体（CC）进行了肽组学分析。与对照组相比，在 MS 小鼠模型中鉴定出总共 217 种肽以不同水平表达。通过进行 GO（基因本体论）和 KEGG（京都基因与基因组百科全书）分析，我们发现这些差异表达肽（DEPs）的前体蛋白与髓鞘鞘和氧化磷酸化有关。我们的研究是 MS 小鼠模型的第一个脑肽组学研究，揭示了脱髓鞘脑组织中 DEP 的独特特征。这些 DPEs 可能为 MS 的发病机制和复杂性提供进一步的深入了解，这将有助于发现治疗 MS 的潜在新的有效策略。
