Bouassida Mouna, Mnif Inès, Hammami Ines, Triki Mohamed-Ali, Ghribi Dhouha
Laboratoire d'Amélioration des Plantes et Valorisation des Agro-Ressources, Ecole Nationale d'Ingénieurs de Sfax, Sfax, Tunisie.
Bioréacteur couple à un ultra filtre, Ecole Nationale D'Ingénieurs de Sfax, Sfax, Tunisie.
Food Sci Biotechnol. 2023 Mar 6;32(11):1595-1609. doi: 10.1007/s10068-023-01274-5. eCollection 2023 Oct.
SPB1 derived biosurfactants (BioS) proved its bio-control activity against using tomato plant. Almost 83% of disease symptoms triggered by were reduced. Aiming potential application, we studied lipopeptide cost-effective production in both fermentations systems, namely the submerged fermentation (SmF) and the solid-state fermentation (SSF) as well as the use of Aleppo pine waste and confectionery effluent as cheap substrates. Optimization studies using Box-Behnken (BB) design followed by the analysis with response surface methodology were applied. When using an effluent/sea water ratio of 1, Aleppo pine waste of 14.08 g/L and an inoculum size of 0.2, a best production yield of 17.16 ± 0.91 mg/g was obtained for the SmF. While for the SSF, the best production yield of 27.59 ± 1.63 mg/g was achieved when the value of Aleppo pine waste, moisture, and inoculum size were, respectively, equal to 25 g, 75%, and 0.2. Hence, this work demonstrated the superiority of SSF over SmF.
源自SPB1的生物表面活性剂（BioS）在番茄植株上证明了其对[病原体名称缺失]的生物防治活性。由[病原体名称缺失]引发的疾病症状减少了近83%。为了实现潜在应用，我们研究了在两种发酵系统中脂肽的经济高效生产，即深层发酵（SmF）和固态发酵（SSF），以及使用阿勒颇松废料和糖果废水作为廉价底物。采用了Box-Behnken（BB）设计进行优化研究，随后用响应面法进行分析。在深层发酵中，当废水/海水比例为1、阿勒颇松废料为14.08 g/L且接种量为0.2时，获得了17.16±0.91 mg/g的最佳产量。而对于固态发酵，当阿勒颇松废料、湿度和接种量分别为25 g、75%和0.2时，实现了27.59±1.63 mg/g的最佳产量。因此，这项工作证明了固态发酵优于深层发酵。
