Lee Da-Young, Song Moon-Young, Hong Kyung-Sook, Yun Sun-Mi, Han Young-Min, Kim Eun-Hee
College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam, 13488 Republic of Korea.
Food Sci Biotechnol. 2023 Apr 4;32(11):1551-1559. doi: 10.1007/s10068-023-01278-1. eCollection 2023 Oct.
Gastric mucosa is important to protect the gastric damage against external factors. We previously reported the gastro-protective effects of steamed and freeze-dried mature silkworm larval powder (SMSP) in ethanol-treated rats. However, the factors that promote mucosal formation still remain unclarified. In this study, we evaluated the effect of SMSP on the restoration and maintenance of gastric mucosal layer as well as anti-inflammatory response in ethanol-induced stomach injury in rats. A significant decrease of ulcer indexes, histopathological scores and pro-inflammatory cytokine levels was observed in SMSP-treated group. In addition, SMSP protected the mucosal layer from ethanol-induced gastric damage by increasing the expression of nitric oxide synthases and heat shock proteins, along with promoting genes related gastric mucosal protection and biosynthesis including mucin 5AC and trefoil factors. These results demonstrate that SMSP attenuates the pro-inflammatory responses and strengthens the gastric mucosal layer, thus exhibiting gastro-protective effect against ethanol-induced gastric injury in rats.
胃黏膜对于保护胃部免受外部因素的损伤十分重要。我们之前报道过，蒸制和冻干的成熟蚕幼虫粉（SMSP）对乙醇处理的大鼠具有胃保护作用。然而，促进黏膜形成的因素仍不明确。在本研究中，我们评估了SMSP对乙醇诱导的大鼠胃损伤中胃黏膜层的修复和维持以及抗炎反应的影响。在SMSP处理组中，溃疡指数、组织病理学评分和促炎细胞因子水平均显著降低。此外，SMSP通过增加一氧化氮合酶和热休克蛋白的表达，以及促进与胃黏膜保护和生物合成相关的基因（包括黏蛋白5AC和三叶因子），保护黏膜层免受乙醇诱导的胃损伤。这些结果表明，SMSP可减轻促炎反应并强化胃黏膜层，从而对乙醇诱导的大鼠胃损伤表现出胃保护作用。
