Park Chae-Eun, Lee Syng-Ook
Department of Food Science and Technology, Keimyung University, Daegu, 42601 Republic of Korea.
Food Sci Biotechnol. 2023 Feb 27;32(11):1561-1571. doi: 10.1007/s10068-023-01279-0. eCollection 2023 Oct.
In this study, we hypothesized that larvae (PBL) protein hydrolysates, which have been previously reported to exhibit strong antioxidant activity, might protect liver cells against oxidative stress-induced injury. Thus, the cytoprotective effects and related mechanisms of PBL hydrolysates were investigated in AML12 liver cells. Among PBL hydrolysates prepared by three different proteases, the PBL flavouryzme hydrolysate showed significantly higher protective effect against HO-induced cytotoxicity than other hydrolysates in AML12 cells. Further mechanistic studies showed that pretreatment with PFH reduces cellular level of reactive oxygen species through induction of Nrf2-mediated antioxidant enzymes such as catalase and heme oxygenase-1. In addition, the free amino acid analysis revealed that PFH was rich in branched-chain amino acids, aromatic amino acids, and hydrophobic amino acids as compared to other hydrolysates, which could contribute to its hepatoprotective effect. These findings suggest that PFH represents a potential source of nutraceuticals that supports liver functions.
The online version contains supplementary material available at 10.1007/s10068-023-01279-0.
在本研究中，我们假设先前报道具有强抗氧化活性的幼虫（PBL）蛋白水解物可能保护肝细胞免受氧化应激诱导的损伤。因此，在AML12肝细胞中研究了PBL水解物的细胞保护作用及相关机制。在由三种不同蛋白酶制备的PBL水解物中，PBL风味酶水解物在AML12细胞中对HO诱导的细胞毒性显示出比其他水解物显著更高的保护作用。进一步的机制研究表明，用PFH预处理通过诱导Nrf2介导的抗氧化酶如过氧化氢酶和血红素加氧酶-1来降低细胞内活性氧水平。此外，游离氨基酸分析显示，与其他水解物相比，PFH富含支链氨基酸、芳香族氨基酸和疏水氨基酸，这可能有助于其肝脏保护作用。这些发现表明，PFH是一种支持肝功能的潜在营养保健品来源。
在线版本包含可在10.1007/s10068-023-01279-0获取的补充材料。
