Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Vet Clin Pathol. 2023 Sep;52(3):422-432. doi: 10.1111/vcp.13228. Epub 2023 Aug 28.
The regenerative response following Babesia rossi infection in dogs is mild, despite severe hemolytic anemia.
We aimed to compare the admission absolute reticulocyte count (ARC) and reticulocyte indices in 103 dogs naturally infected with B. rossi with 10 dogs suffering from immune-mediated hemolytic anemia (IMHA) and 14 healthy control dogs. The regenerative response was also evaluated in five dogs experimentally infected with B. rossi.
This is a retrospective observational study of records generated on the ADVIA 2120 hematology analyzer.
The median hematocrits (HCT) of the B. rossi and IMHA groups were significantly lower than the control group (p < .001 for both); however, no differences were seen between the B. rossi and IMHA groups. Compared with the control group, the median ARC was significantly higher in the B. rossi (p = .006) and IMHA (p = .019) groups but significantly lower in the B. rossi group than the IMHA group (p = .041). In the experimentally infected dogs, there was a sudden decrease in the ARC approximately 48 h after the detection of peripheral parasitemia, which was followed by an increase after treatment. Reticulocytes of naturally infected B. rossi dogs were larger, with more variation in cellular volume. The reticulocytes of the experimentally infected dogs decreased in size with decreasing hemoglobin concentrations as the study progressed.
The regenerative response in dogs naturally infected with B. rossi is inadequate, given the severity of the anemia observed, and it might be a result of direct suppressive action by the parasite or host response on the bone marrow.
尽管犬巴贝斯虫感染会导致严重的溶血性贫血，但再生反应较为温和。
我们旨在比较 103 只自然感染巴贝斯虫的犬、10 只免疫介导性溶血性贫血（IMHA）犬和 14 只健康对照犬的入院时绝对网织红细胞计数（ARC）和网织红细胞指数，并评估 5 只经实验感染巴贝斯虫的犬的再生反应。
这是一项回顾性观察研究，记录了 ADVIA 2120 血液分析仪生成的数据。
巴贝斯虫和 IMHA 组的中位数血细胞比容（HCT）明显低于对照组（均 < .001）；然而，巴贝斯虫和 IMHA 组之间没有差异。与对照组相比，巴贝斯虫（p = .006）和 IMHA 组（p = .019）的中位数 ARC 明显更高，但巴贝斯虫组明显低于 IMHA 组（p = .041）。在实验感染的犬中，在外周寄生虫血症检出后约 48 h，ARC 突然下降，随后在治疗后增加。自然感染巴贝斯虫的犬的网织红细胞较大，细胞体积变化较大。随着研究的进行，实验感染犬的网织红细胞体积减小，血红蛋白浓度降低。
鉴于观察到的贫血严重程度，自然感染犬的再生反应不足，这可能是寄生虫或宿主对骨髓的直接抑制作用的结果。
