Celliers Anri, Rautenbach Yolandi, Hooijberg Emma, Christopher Mary, Goddard Amelia
Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
Front Vet Sci. 2020 Feb 18;7:72. doi: 10.3389/fvets.2020.00072. eCollection 2020.
Babesiosis caused by the virulent tick-borne hemoprotozoan, , results in a marked systemic inflammatory host response in dogs. Neutrophils form part of the innate immune response and contains myeloperoxidase (MPO) as the predominant component of the neutrophil lysosomal protein in azurophilic granules. The neutrophil myeloperoxidase index (MPXI), determined on the ADVIA hematology analyzer, is a quantitative estimate of intracellular MPO content. Objectives of this study were to: (a) compare MPXI in dogs with babesiosis with healthy control dogs; (b) compare MPXI in dogs that died from babesiosis with dogs that survived and controls; and (c) correlate the MPXI with the previously determined segmented and band neutrophil count and cytokine concentrations in dogs with babesiosis. Data for 140 dogs naturally infected with and 20 healthy control dogs were retrospectively evaluated. Neutrophil counts and MPXI were determined on an ADVIA 2120 analyzer. Cytokine concentrations [interleukin (IL)-2, IL-6, IL-8, IL-10, IL-18, granulocyte-macrophage colony stimulating factor (GM-CSF), and monocyte chemo-attractant protein-1 (MCP-1)] were determined using a canine-specific multiplex immunoassay. The mortality rate of the dogs was 11% (15/140). MPXI was significantly higher in dogs ( = 0.033), and in non-survivors ( = 0.011), compared with healthy control dogs. In dogs a significant positive correlation was found between MPXI and IL-10 ( = 0.211, = 0.039) and a significant negative correlation was found between MPXI and IL-8 ( = -0.350, < 0.001). In non-survivors, significant positive correlations were found between MPXI and IL-2 ( = 0.616 = 0.033), IL-6 ( = 0.615, = 0.033), IL-18 ( = 0.613, = 0.034), GM-CSF ( = 0.630, = 0.028), and MCP-1 ( = 0.713, = 0.009). In survivors, a significant negative correlation was found between MPXI and IL-8 ( = -0.363, = 0.001). MPXI was correlated with pro-inflammatory cytokines in dogs that died. The potential of MPXI as a novel marker of inflammation and prognosis in dogs infected with , thus warrants further investigation.
由毒性蜱传血原虫引起的巴贝斯虫病会在犬类中引发明显的全身性炎症宿主反应。中性粒细胞是固有免疫反应的一部分，其嗜天青颗粒中含有髓过氧化物酶（MPO）作为中性粒细胞溶酶体蛋白的主要成分。在ADVIA血液分析仪上测定的中性粒细胞髓过氧化物酶指数（MPXI）是细胞内MPO含量的定量估计。本研究的目的是：（a）比较患巴贝斯虫病的犬与健康对照犬的MPXI；（b）比较死于巴贝斯虫病的犬与存活犬及对照犬的MPXI；（c）将患巴贝斯虫病犬的MPXI与先前测定的分叶核和杆状核中性粒细胞计数及细胞因子浓度进行关联。对140只自然感染的犬和20只健康对照犬的数据进行了回顾性评估。在ADVIA 2120分析仪上测定中性粒细胞计数和MPXI。使用犬特异性多重免疫测定法测定细胞因子浓度[白细胞介素（IL）-2、IL-6、IL-8、IL-10、IL-18、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和单核细胞趋化蛋白-1（MCP-1）]。感染犬的死亡率为11%（15/140）。与健康对照犬相比，患巴贝斯虫病犬的MPXI显著更高（P = 0.033），且非存活犬的MPXI也显著更高（P = 0.011）。在患巴贝斯虫病犬中，发现MPXI与IL-10之间存在显著正相关（r = 0.211，P = 0.039），与IL-8之间存在显著负相关（r = -0.350，P < 0.001）。在非存活犬中，发现MPXI与IL-2（r = 0.616，P = 0.033）、IL-6（r = 0.615，P = 0.033）、IL-18（r = 0.613，P = 0.034）、GM-CSF（r = 0.630，P = 0.028）和MCP-1（r = 0.713，P = 0.009）之间存在显著正相关。在存活犬中，发现MPXI与IL-8之间存在显著负相关（r = -0.363，P = 0.001）。在死于巴贝斯虫病的犬中，MPXI与促炎细胞因子相关。因此，MPXI作为感染犬炎症和预后新标志物的潜力值得进一步研究。
