Saakyan S V, Myakoshina E B, Tatskov R A, Katargina L A, Panova A Yu
Helmholtz National Medical Research Center of Eye Diseases, Moscow, Russia.
Vestn Oftalmol. 2023;139(4):5-11. doi: 10.17116/oftalma20231390415.
Optical coherence tomography angiography (OCTA) is a non-invasive diagnostic method used in children and adults. Features of angioarchitecture of small retinoblastoma are not sufficiently covered.
The study investigated the angioarchitecture of small retinoblastomas using OCTA.
The study included 10 children with binocular retinoblastoma aged 2.7±0.5 months with small tumors of central localization (10 foci). The tumors were divided into 3 groups: group 1 (=4) - tumor thickness 0.8±0.2 mm; group 2 (=3) - 1.6±0.5 mm; group 3 (=3) - 2.4±0.8 mm. OCTA was performed on Spectralis HRA+OCT (2460 scans in total). Vessels were identified in the superficial, deep and outer layers of the tumor on En Face images. Their average number was estimated by visualization of yellow pixels in the superficial layers on 10 sagittal sections. Statistical analysis was done using Microsoft Excel, Statistica 8.0. The Kruskal-Wallis H test was used for comparative analysis of independent variables with more than two samples.
Retinal vessels with feeding anastomoses connecting them to multiple small tortuous tumor vessels in the superficial layers were identified in group 1. Number of yellow pixels - 16.5±0.5. In the deep layers - single chaotic vascular arcades. In flat small retinoblastomas the vascular component was not evaluated. In group 2 in the superficial layers of the tumor we found multiple geniculate vessels of large and small caliber anastomosing between themselves and the retinal vessels. Number of yellow pixels was 21±0.8. A few vessels were identified in the deep and outer layers. In group 3 we identified single convoluted vessels in the superficial layers with glow and quantity increasing in the deep layers. In the deep layers - emergence of a small number of vessels. The maximum number of multiple own tumor vessels was determined in the outer layers. Number of yellow pixels - 10±0.8.
The obtained results confirm the possibility to preclinically identify the angioarchitecture of small retinoblastomas in order to determine the activity of tumor growth and serve as a marker of neoplasm regression in the future, after organ-preserving treatment.
光学相干断层扫描血管造影（OCTA）是一种用于儿童和成人的非侵入性诊断方法。小视网膜母细胞瘤的血管结构特征尚未得到充分研究。
本研究使用OCTA研究小视网膜母细胞瘤的血管结构。
本研究纳入10例双眼视网膜母细胞瘤患儿，年龄2.7±0.5个月，肿瘤位于中央，体积较小（10个病灶）。将肿瘤分为3组：第1组（=4）-肿瘤厚度0.8±0.2mm；第2组（=3）-1.6±0.5mm；第3组（=3）-2.4±0.8mm。使用Spectralis HRA+OCT进行OCTA检查（共扫描2460次）。在En Face图像上识别肿瘤浅层、深层和外层的血管。通过观察10个矢状面上浅层的黄色像素来估计其平均数量。使用Microsoft Excel、Statistica 8.0进行统计分析。采用Kruskal-Wallis H检验对两个以上样本的自变量进行比较分析。
第1组在浅层发现视网膜血管通过供血吻合与多个细小迂曲的肿瘤血管相连。黄色像素数量-16.5±0.5。深层为单个混乱的血管弓。扁平小视网膜母细胞瘤未评估血管成分。第2组在肿瘤浅层发现多个大小口径的膝状血管相互吻合，并与视网膜血管相连。黄色像素数量为21±0.8。在深层和外层发现少量血管。第3组在浅层发现单个盘绕血管，有光晕，深层数量增加。深层出现少量血管。在外层确定了最多数量的多个自身肿瘤血管。黄色像素数量-10±0.8。
所得结果证实了在临床前识别小视网膜母细胞瘤血管结构的可能性，以便确定肿瘤生长活性，并在未来保器官治疗后作为肿瘤消退的标志物。
